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Information Journal Paper

Title

MYCOPROTEIN PRODUCTION BY FUSARIUM VENENATUM IN SURFACE CULTURE USING A CENTRAL COMPOSITE DESIGN

Pages

  45-52

Abstract

 Background and Objectives: Due to their nutritional, physical and chemical characteristics, MYCOPROTEINs, produced in the process of fungal growth, have been proposed to be used as a food additive for man. In this study a CENTRAL COMPOSITE DESIGN was applied to assessing the effect of seed size and initial concentrations of carbon and nitrogen sources on MYCOPROTEIN production by FUSARIUM VENENATUM (ATCC20334) from date sugar. Materials and methods: Chemical composition (percent dried cell weight, nitrogen, moisture, and ash) and pH of the substrate were determined. Vogel culture medium was used for maintenance and inoculums preparation, and incubation was done at 30°C for 72h. Dried cell weight was measured by the oven method. The experimental results were fitted to a full quadratic second order polynomial equation and the optimization of response was done with Minitab 14 software. Optimum conditions were determined and a combination test was conducted.Results: The regression coefficient of the model presented for MYCOPROTEIN production (based on dried cell weight) was 98.0%. The optimum conditions for the MYCOPROTEIN production included a seed size of 15.39 % v/v, and a carbon and nitrogen source of 20 and 4.35 g/L, respectively. The predicted value for the optimum production of dried biomass was 5.032 g/L. The recombination test was conducted at the optimum conditions and the result obtained was 4.84 g/L of biomass production. The regression coefficient indicates accuracy and suitability of the model for the experimental data.Conclusion: By changing the inoculums conditions and the chemical composition of the culture medium, optimal growth of FUSARIUM VENENATUM and, consequently, maximum biomass production, can be achieved, Use of date syrup as an available and domestic Iranian substrate for modification of Vogel medium makes possible protein production at an industrial scale in SURFACE CULTURE.

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    APA: Copy

    HOSSEINI, SEYED MARZIEH, KHOSRAVI DARANI, KIANOUSH, MOHAMMADIFAR, M.A., NIKOUPOUR, H., HOSSEINI, HEDAYAT, & VALAEI, NASER. (2010). MYCOPROTEIN PRODUCTION BY FUSARIUM VENENATUM IN SURFACE CULTURE USING A CENTRAL COMPOSITE DESIGN. JOURNAL OF NUTRITION SCIENCES & FOOD TECHNOLOGY, 4(4 (15)), 45-52. SID. https://sid.ir/paper/121506/en

    Vancouver: Copy

    HOSSEINI SEYED MARZIEH, KHOSRAVI DARANI KIANOUSH, MOHAMMADIFAR M.A., NIKOUPOUR H., HOSSEINI HEDAYAT, VALAEI NASER. MYCOPROTEIN PRODUCTION BY FUSARIUM VENENATUM IN SURFACE CULTURE USING A CENTRAL COMPOSITE DESIGN. JOURNAL OF NUTRITION SCIENCES & FOOD TECHNOLOGY[Internet]. 2010;4(4 (15)):45-52. Available from: https://sid.ir/paper/121506/en

    IEEE: Copy

    SEYED MARZIEH HOSSEINI, KIANOUSH KHOSRAVI DARANI, M.A. MOHAMMADIFAR, H. NIKOUPOUR, HEDAYAT HOSSEINI, and NASER VALAEI, “MYCOPROTEIN PRODUCTION BY FUSARIUM VENENATUM IN SURFACE CULTURE USING A CENTRAL COMPOSITE DESIGN,” JOURNAL OF NUTRITION SCIENCES & FOOD TECHNOLOGY, vol. 4, no. 4 (15), pp. 45–52, 2010, [Online]. Available: https://sid.ir/paper/121506/en

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