ACCURACY OF IDENTIFICATION AND SUSCEPTIBILITY TESTS OF GRAM NEGATIVE BACILLI PERFORMED ROUTINELY BY CLINICAL LABORATORIES COMPARED TO THE STANDARD PROCEDURES IN KERMAN

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ABBASI SAMANEH; MANSOURI SHAHLA

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Paper Information

Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2008 | Volume:2 | Issue:2 Page(s): 49-54

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Title

ACCURACY OF IDENTIFICATION AND SUSCEPTIBILITY TESTS OF GRAM NEGATIVE BACILLI PERFORMED ROUTINELY BY CLINICAL LABORATORIES COMPARED TO THE STANDARD PROCEDURES IN KERMAN

Author(s)

ABBASI SAMANEH | MANSOURI SHAHLA | Issue Writer Certificate

Keywords

ENTERIC BACILLIQ4

LABORATORY REPORTSQ4

IDENTIFICATIONQ3

DRUG RESISTANCEQ3

Abstract

Background and aim: Gram -negative rods are the most common bacterial isolates in clinical laboratories around the word. Quick and accurate IDENTIFICATION of these bacteria is a key to the effective therapeutic intervention and optimal clinical outcome of infections caused by these bacteria. Multiple DRUG RESISTANCE gram-negative bacteria (MDR) are on increase and are reported frequently. Since in many clinical laboratories the standard antibiogram tests are not performed and very few tests are used for IDENTIFICATION. The aim of this study was to evaluate the accuracy of IDENTIFICATION and the susceptibility tests of gram negative bacilli performed by clinical laboratories compared to the standard procedures in Kerman.Materials and Methods: This study was performed on 948 clinical isolates reported to have the MDR phenotypes by disk diffusion method. The isolates were identified to species level by conventional biochemical tests. Sensitivity of the isolates to antibacterial agents and the Minimum Inhibitory concentration (MIC) of the isolates were determined using agar dilution method. For statistical analysis, x2 test was used.Results: The rate of isolation of MDR bacteria by agar dilution methods was 76% of those reported by disk diffusion methods. For all antibiotics disk diffusion showed higher rate of resistance compared with agar dilution. The difference in case of tetracycline, gentamicin, ciprofloxacin, amoxicillin and cephalosporins (ceftizoxime, ceftazidime) and trimethoprime-sulfamethoxazole were significant (P=0.002). In the centers under study lower rate was reported for the isolation of Enterobacter spp and citrobacter spp, while Escherichia coli and Klebsiella spp were reported more than their actual presence.Conclusion: These results showed that the LABORATORY REPORTS were relatively accurate. However since higher rate of bacterial resistance were reported by the disk diffusion methods, in case of serious and life threatening infections suitable drug for treatment should be confirmed by more sensitive tests. In the absence of suitable and low cost detection kits for the IDENTIFICATION of gram negative bacilli, inaccurate IDENTIFICATIONs is an avoidable, therefore immediate action to design the accurate and low cost kits for the IDENTIFICATION of these bacteria is recommended in the country.

Cites

SURVEY OF ANTIBACTERIAL AND ANTIFUNGAL IMPACT OF ESSENTIAL OIL OF ARTEMISIA KERMANENSIS

References

PREVALENCE OF GROUP-BSTREPTOCOCCAL COLONIZATION IN VAGINA AND RECTUM OF 35-37 WEEKS PREGNANT WOMEN AND ITS SENSITIVITY TO ANTIBIOTICS

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APA: Copy

ABBASI, SAMANEH, & MANSOURI, SHAHLA. (2008). ACCURACY OF IDENTIFICATION AND SUSCEPTIBILITY TESTS OF GRAM NEGATIVE BACILLI PERFORMED ROUTINELY BY CLINICAL LABORATORIES COMPARED TO THE STANDARD PROCEDURES IN KERMAN. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2(2), 49-54. SID. https://sid.ir/paper/132617/en

Vancouver: Copy

ABBASI SAMANEH, MANSOURI SHAHLA. ACCURACY OF IDENTIFICATION AND SUSCEPTIBILITY TESTS OF GRAM NEGATIVE BACILLI PERFORMED ROUTINELY BY CLINICAL LABORATORIES COMPARED TO THE STANDARD PROCEDURES IN KERMAN. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2008;2(2):49-54. Available from: https://sid.ir/paper/132617/en

IEEE: Copy

SAMANEH ABBASI, and SHAHLA MANSOURI, “ACCURACY OF IDENTIFICATION AND SUSCEPTIBILITY TESTS OF GRAM NEGATIVE BACILLI PERFORMED ROUTINELY BY CLINICAL LABORATORIES COMPARED TO THE STANDARD PROCEDURES IN KERMAN,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 2, no. 2, pp. 49–54, 2008, [Online]. Available: https://sid.ir/paper/132617/en

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