Unlocking Therapeutic Potential: Enhanced shRNA Delivery with Tat Peptide in the Human Respiratory Syncytial Virus Treatment

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Amiri Zadeh Fard Saeid; Abuei Haniyeh; Behzad Behbahani Abbas; Rafiei Dehbidi Gholamreza; Zare Farahnaz; Nejabat Maryam; Safarpour Alireza; Farhadi Ali

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Journal Paper

Paper Information

Journal: Advanced Pharmaceutical Bulletin Year:2024 | Volume:14 | Issue:3 Page(s): 696-704

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Title

Unlocking Therapeutic Potential: Enhanced shRNA Delivery with Tat Peptide in the Human Respiratory Syncytial Virus Treatment

Author(s)

Keywords

Keywords: Fusion geneQ4

Matrix geneQ4

Human respiratory syncytial virusQ4

Real-time PCRQ4

RibavirinQ4

shRNAQ4

Tat peptideQ4

Abstract

Purpose: This research investigated the development of short hairpin RNA (shRNA) molecules designed to target specific regions of the Human respiratory syncytial virus (HRSV) M and F genes. The study aimed to assess the therapeutic potential of these shRNAs and evaluate the effectiveness of Tat peptide-mediated delivery in enhancing their functionality. Methods: We acquired isolates from pediatric patients experiencing respiratory illness then cultured in HEp-2 cells. We constructed plasmids expressing shRNAs. Tat peptide as a facilitator for shRNA plasmid delivery was used. The cytotoxicity of Ribavirin, shRNA constructs, and control agents was assessed using the MTT assay. The transfection efficiency of Tat peptide-mediated shRNA delivery with that of lipofectamine 3000™ were compared. Finally, Real-time PCR was employed to quantify HRSV replication in the treated cells. Results: Tat peptide-mediated delivery of shRNA plasmids significantly suppressed the expression of the M and F genes of HRSV compared to lipofectamine 3000™. This suppression was evident in both short-term experiments and scenarios involving stable shRNA expression. Furthermore, the combination of Ribavirin with shRNA treatment resulted in a substantial reduction in viral load. Notably, the most pronounced antiviral effect was observed when both shRNAs were employed simultaneously. Conclusion: Our findings suggest that Tat peptide-mediated delivery of shRNA plasmids holds significant potential for achieving stable suppression of HRSV genes. This approach warrants further investigation as a potential gene therapy strategy for HRSV. By demonstrating promising results in vitro, this study highlights the need for future in vivo studies to comprehensively evaluate the therapeutic potential of this approach in a clinical setting.

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