DESIGN AND CONSTRUCTION OF E2-FLIC CONSTRUCT USING FLIC GENE FROM SALMONELLA ENTERICA AND E2 GENE FROM HEPATITIS C VIRUS AND ITS EXPRESSION IN EUKARYOTIC SYSTEM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SOLTAN DALLAL MOHAMMAD MAHDI; KESHAVARZ ABOLFAZL; KORD EBRAHIM; ANSARI NASTARAN; HAJIKHEZRI ZAMANEH; SAMIMI RAD KATAYOUN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Payavard Salamat Year:2018 | Volume:12 | Issue:1 Page(s): 1-10

Download Full-Text

Persian Verion

View:

801

Download:

Cites:

Information Journal Paper

Title

DESIGN AND CONSTRUCTION OF E2-FLIC CONSTRUCT USING FLIC GENE FROM SALMONELLA ENTERICA AND E2 GENE FROM HEPATITIS C VIRUS AND ITS EXPRESSION IN EUKARYOTIC SYSTEM

Author(s)

Keywords

FLIC GENEQ1

SALMONELLA ENTERICAQ1

E2 GENEQ1

HEPATITIS C VIRUSQ1

EXPRESSION OF E2-FLICQ1

FUSION PROTEINQ1

Abstract

Background and Aim: At the present time one of the strategies in vaccine design is generation of FUSION PROTEINs containing (including) immunogen of infectious agents and adjuvants. In this study design and construction of E2-fliC fragment as a vaccine candid was conducted by using FLIC GENE from SALMONELLA ENTERICA and E2 GENE from HEPATITIS C VIRUS.Materials and Methods: To prepare the E2-fliC construct, E2 and fliC fragments were first amplified from pBluscript-E2 and pBluscript-fliC, respectively by PCR method. To generate pcDNA-E2-fliC plasmid, E2 was subcloned into plasmid pcDNA3.1 (+) which was extracted from DH5a cells. ThefliC sequence was then cloned into the pcDNA3.1-E2. To evaluate the EXPRESSION OF E2-FLIC construct, it was inserted into the pEGFP-N3 expression vector. Then COS-7 cells were transfected with pEGFPN3- E2-fliC to evaluate the expression of the FUSION PROTEIN by observation of the EGFP signal under the fluorescence microscope.Results: By development of GFP fluorescent using fluorescence microscopy the most EXPRESSION OF E2-FLIC construct was observed at 24h after transfection. The accuracy of the recombinant plasmid pcDNA-E2-fliC was confirmed by PCR, restriction enzymes and DNA sequencing.Conclusion: Our findings suggest that E2-FliC FUSION PROTEIN has expressed efficiently and most likely similar to HCV E2 protein induces immune system of mice after their immunization with pcDNA-E2-fliC.

Cites

No record.

References

core domain of Hepatitis C Virus glycoprotein E2 generates potent cross-neutralizing antibodies in guinea pigs

Cite

APA: Copy

SOLTAN DALLAL, MOHAMMAD MAHDI, KESHAVARZ, ABOLFAZL, KORD, EBRAHIM, ANSARI, NASTARAN, HAJIKHEZRI, ZAMANEH, & SAMIMI RAD, KATAYOUN. (2018). DESIGN AND CONSTRUCTION OF E2-FLIC CONSTRUCT USING FLIC GENE FROM SALMONELLA ENTERICA AND E2 GENE FROM HEPATITIS C VIRUS AND ITS EXPRESSION IN EUKARYOTIC SYSTEM. PAYAVARD-SALAMAT, 12(1 ), 1-10. SID. https://sid.ir/paper/149621/en

Vancouver: Copy

SOLTAN DALLAL MOHAMMAD MAHDI, KESHAVARZ ABOLFAZL, KORD EBRAHIM, ANSARI NASTARAN, HAJIKHEZRI ZAMANEH, SAMIMI RAD KATAYOUN. DESIGN AND CONSTRUCTION OF E2-FLIC CONSTRUCT USING FLIC GENE FROM SALMONELLA ENTERICA AND E2 GENE FROM HEPATITIS C VIRUS AND ITS EXPRESSION IN EUKARYOTIC SYSTEM. PAYAVARD-SALAMAT[Internet]. 2018;12(1 ):1-10. Available from: https://sid.ir/paper/149621/en

IEEE: Copy

MOHAMMAD MAHDI SOLTAN DALLAL, ABOLFAZL KESHAVARZ, EBRAHIM KORD, NASTARAN ANSARI, ZAMANEH HAJIKHEZRI, and KATAYOUN SAMIMI RAD, “DESIGN AND CONSTRUCTION OF E2-FLIC CONSTRUCT USING FLIC GENE FROM SALMONELLA ENTERICA AND E2 GENE FROM HEPATITIS C VIRUS AND ITS EXPRESSION IN EUKARYOTIC SYSTEM,” PAYAVARD-SALAMAT, vol. 12, no. 1 , pp. 1–10, 2018, [Online]. Available: https://sid.ir/paper/149621/en

Related Journal Papers