EFFECT OF DIFFERENT PRESERVATIVES DURING FREEZING PROCESS ON INFECTIVITY OF LEISHMANIA MAJOR IN MICE MODEL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ZARRINKAR FARZANEH; KHAMESIPOUR ALI; MIRAMIN MOHAMMADI AKRAM; ESKANDARI SEYED EBRAHIM; NATEGHI ROSTAMI MAHMOUD; FALAH ESMAEIL

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Dermatology and Cosmetics Year:2012 | Volume:3 | Issue:3 Page(s): 125-133

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

697

Download:

Cites:

Information Journal Paper

Title

EFFECT OF DIFFERENT PRESERVATIVES DURING FREEZING PROCESS ON INFECTIVITY OF LEISHMANIA MAJOR IN MICE MODEL

Author(s)

Keywords

LEASHMANIAQ3

LEISHMANIA MAJORQ3

VIRULENCEQ2

BALB/C MOUSEQ2

Abstract

Background and Aim: Leishmanization (LZ) is an effective tool to prevent cutaneous leishmaniasis. Standardization of Leishmaniais the main drawback of LZ. The aim of this study was to assess the effect of various preservatives on the infectivity of Leishmania.Methods: L.major harvested at different stages of growth; logarithmic, early and late stationary phases were frozen using various preservatives of saccharose, glycerol, trehalose, glucose, sorbitol, and dimethyl sulfoxide (DMSO). The harvested parasites were inoculated into BALB/c mice before and after freezing. The infectivity of the parasites was checked. IFA test was used to assess the rate of metacyclic parasite.Results: The ratio of live Leishmania in different growth stages and various preservatives were 89.0% to 98.2%. The lesion development in groups of mice which received Leishmaniain sacarose+glycerol or DMSO was started from 3rd week and at 5th week all the mice showed lesion. The group of mice which were inoculated with early or late stationary phases in saccharose+glucose, saccharose+ glycerol, glycerol 15% or DMSO showed lesion from 4th to 5th week and in 100% showed lesions at 8th week. The rate of metacyclic parasites increases from log phase to early and late stationary phases.Conclusion: There was a correlation between percent of live parasite and the rate of lesion development in BALB/c mice. Saccharose 22.5%+Glyserol 22.5% were the most appropriate preservative to freezeL. major. IFA test is used to detect metacyclicLeishmania. A correlation was seen between the rate of lesion development in BALB/c mice and IFA positivity.

Cites

No record.

References

No record.

Cite

APA: Copy

ZARRINKAR, FARZANEH, KHAMESIPOUR, ALI, MIRAMIN MOHAMMADI, AKRAM, ESKANDARI, SEYED EBRAHIM, NATEGHI ROSTAMI, MAHMOUD, & FALAH, ESMAEIL. (2012). EFFECT OF DIFFERENT PRESERVATIVES DURING FREEZING PROCESS ON INFECTIVITY OF LEISHMANIA MAJOR IN MICE MODEL. DERMATOLOGY AND COSMETIC, 3(3), 125-133. SID. https://sid.ir/paper/157696/en

Vancouver: Copy

ZARRINKAR FARZANEH, KHAMESIPOUR ALI, MIRAMIN MOHAMMADI AKRAM, ESKANDARI SEYED EBRAHIM, NATEGHI ROSTAMI MAHMOUD, FALAH ESMAEIL. EFFECT OF DIFFERENT PRESERVATIVES DURING FREEZING PROCESS ON INFECTIVITY OF LEISHMANIA MAJOR IN MICE MODEL. DERMATOLOGY AND COSMETIC[Internet]. 2012;3(3):125-133. Available from: https://sid.ir/paper/157696/en

IEEE: Copy

FARZANEH ZARRINKAR, ALI KHAMESIPOUR, AKRAM MIRAMIN MOHAMMADI, SEYED EBRAHIM ESKANDARI, MAHMOUD NATEGHI ROSTAMI, and ESMAEIL FALAH, “EFFECT OF DIFFERENT PRESERVATIVES DURING FREEZING PROCESS ON INFECTIVITY OF LEISHMANIA MAJOR IN MICE MODEL,” DERMATOLOGY AND COSMETIC, vol. 3, no. 3, pp. 125–133, 2012, [Online]. Available: https://sid.ir/paper/157696/en

Related Journal Papers