DEVELOPMENTAL POTENTIAL OF MOUSE MORULA EARLY AND LATE BLASTOCYST AFTER VITRIFICATION

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EZATABADIPOUR M.; HOSSEINI A.; BAHARVAND H.; NEMATOLAHI S.N.; HEYDARI M.H.

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Journal: Cell Journal (Yakhteh) Year:2002 | Volume:4 | Issue:13 Page(s): 23-32

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Title

DEVELOPMENTAL POTENTIAL OF MOUSE MORULA EARLY AND LATE BLASTOCYST AFTER VITRIFICATION

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Keywords

MORULAQ3

BLASLOCYSTQ3

MOUSEQ2

VITRIFICATIONQ3

CO-CULTUREQ3

Abstract

Introduction: The objective of this study was to assess developmental potential of MOUSE MORULAe, early and laic blastocysts after VITRIFICATION in R2 and R2+Vero mediums. Material and Methods: MORULAe, early and late blastocysts were obtained from superovulated NMRI female mice. Embryos in experimental group were suspended in a solution of Ethylene glycol, ficoll and sucrose (EFS40) for 2 minutes and were then loaded into 0.25 ml straws, holding them on liquid nitrogen vapour for 3-5 minutes, finally were immersed into liquid nitrogen. Embryos were thawed and equilibrated in 0.5 mol sucrose solution for 5 minutes, were cultured in R2 or R2+Vero mediums for I 20 hours. Results: Results indicate high survival rate or embryos after VITRIFICATION (95, 85, 76% for MORULAe, early and late blastocysts respectively). Sixty three, 70 and 75% in R2 and 67, 73, 80% in R2+Vero reached to hatching and hatched blastocyst alter 4.S hours Respectively for MORULAe, early and late blastocyst of control groups. While 24, 19 and 12% in R2, and 18, 22 and 16% in R2+Vero of vitrified embryos were reached to Hatching and hatched blastocyst after 4S hours. Sixty five, 58 and 44% in R2 and 70, 63 and 56% in R2+Vero of vitrified embryos following a delay of 48 hours on the 4th of cultivation day reached lo hatching and hatched blastocyst. Conclusion: Our results indicate that R2 and R2+Vero have the identical property lo support development of vitrified and non-vitrified embryos. Development of vitrified embryos show the same development competence comparing to control group alter a delay of 48 hours, from this statement we conclude that although VITRIFICATION damage the embryos Inn most of these damage are reversible in a suitable medium is less expensive and more convenient than R2+Vero.

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APA: Copy

EZATABADIPOUR, M., HOSSEINI, A., BAHARVAND, H., NEMATOLAHI, S.N., & HEYDARI, M.H.. (2002). DEVELOPMENTAL POTENTIAL OF MOUSE MORULA EARLY AND LATE BLASTOCYST AFTER VITRIFICATION. CELL JOURNAL (YAKHTEH), 4(13), 23-32. SID. https://sid.ir/paper/16179/en

Vancouver: Copy

EZATABADIPOUR M., HOSSEINI A., BAHARVAND H., NEMATOLAHI S.N., HEYDARI M.H.. DEVELOPMENTAL POTENTIAL OF MOUSE MORULA EARLY AND LATE BLASTOCYST AFTER VITRIFICATION. CELL JOURNAL (YAKHTEH)[Internet]. 2002;4(13):23-32. Available from: https://sid.ir/paper/16179/en

IEEE: Copy

M. EZATABADIPOUR, A. HOSSEINI, H. BAHARVAND, S.N. NEMATOLAHI, and M.H. HEYDARI, “DEVELOPMENTAL POTENTIAL OF MOUSE MORULA EARLY AND LATE BLASTOCYST AFTER VITRIFICATION,” CELL JOURNAL (YAKHTEH), vol. 4, no. 13, pp. 23–32, 2002, [Online]. Available: https://sid.ir/paper/16179/en

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