INVESTIGATIONS OF CTPA IN LISTERIA MONOCYTOGENES AND TRANSFER IT TO E.COIL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NOUROUZI JAMILEH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2002 | Volume:4 | Issue:13 Page(s): 5-9

Download Full-Text

Persian Verion

View:

1,048

Download:

Cites:

Information Journal Paper

Title

INVESTIGATIONS OF CTPA IN LISTERIA MONOCYTOGENES AND TRANSFER IT TO E.COIL

Author(s)

NOUROUZI JAMILEH | Issue Writer Certificate

Keywords

LISTERIA MONOCYTOGENESQ3

TRANSFORMATION GENEQ2

CTPA GENEQ3

E.COIL-Α BACTERIAQ3

Abstract

Introduction: LISTERIA MONOCYTOGENES is a gram positive bacteria, frequently found in the environment and is responsible for food-borne disease such as perinatal infections, septicameia and meningeonceohalitis in human and animals.Material and Methods: For this reason, distribution of the ctpA determinate among L. monocytogenes isolated from clinical environment, diry and poultry samples were investigated. Then, CTPA GENE was tranfered into E.coil DH5-α.This investigation was carried out in 2 steps (ctpA was found LISTERIA MONOCYTOGENES isolated from different sources, which was kept in culture collection of Adelaiede University, Australia. Then CTPA GENE was transferred into E.coil DH5-α). CtpA DNA from LISTERIA MONOCYTOGENES was ampilified by PCR, identified on agarose gel, purified by phenol, and ligated into pGEM-T vector. Then transferred on X-gal plate contaning ampicilin. The sequencing of ctpA DNA in with colonies was determined by using by terminator kit and sequencing machine. Results: Using PCR to identify the homologous DNA in 69 isoltes, 38% of isolates tested contained the ctpA determinate. Our results showed that 90% of clinical and dairy isolates, 85% of environmental isolates and 7% of poultry isolates of L. monocytogenes into E.coil DH5-α was successful.Conclusion: Since, the existence of ctpA in clinical, dairy and environmental samples was 90% and in poultry was 7%, so, the virulence of all of strains of this bacteria are not the same, by introducing of such (ctpA gene) into suitable carrier strains, could be expected to producted to produce a good oral immunogen against L. monocytogenes.

Cites

No record.

References

Cite

APA: Copy

NOUROUZI, JAMILEH. (2002). INVESTIGATIONS OF CTPA IN LISTERIA MONOCYTOGENES AND TRANSFER IT TO E.COIL . CELL JOURNAL (YAKHTEH), 4(13), 5-9. SID. https://sid.ir/paper/16185/en

Vancouver: Copy

NOUROUZI JAMILEH. INVESTIGATIONS OF CTPA IN LISTERIA MONOCYTOGENES AND TRANSFER IT TO E.COIL . CELL JOURNAL (YAKHTEH)[Internet]. 2002;4(13):5-9. Available from: https://sid.ir/paper/16185/en

IEEE: Copy

JAMILEH NOUROUZI, “INVESTIGATIONS OF CTPA IN LISTERIA MONOCYTOGENES AND TRANSFER IT TO E.COIL ,” CELL JOURNAL (YAKHTEH), vol. 4, no. 13, pp. 5–9, 2002, [Online]. Available: https://sid.ir/paper/16185/en

Related Journal Papers