CYTOGENETIC EFFECTS OF METHOTREXATE AND CYTARABINE ALONE AND IN COMBINATION ON G1 AND G2 CELL CYCLE STAGES OF LEUKEMIC MOLT-4 CELL LINE IN VITRO

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BARADAR BOKAIE P.

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Journal: Cell Journal (Yakhteh) Year:2003 | Volume:5 | Issue:18 Page(s): 97-101

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Title

CYTOGENETIC EFFECTS OF METHOTREXATE AND CYTARABINE ALONE AND IN COMBINATION ON G1 AND G2 CELL CYCLE STAGES OF LEUKEMIC MOLT-4 CELL LINE IN VITRO

Author(s)

BARADAR BOKAIE P. | Issue Writer Certificate

Keywords

CEL CYCLE STAGEQ3

MOLT-4 CELL LINEQ3

CYTARABINEQ3

METHOREXATEQ3

CHROMOSOMAL ABERRATIONSQ4

Abstract

Introduction: This study was performed to analyze chromosomal aletrations in G1 and G2 immortalized MOLT-4 CELL LINE following treatment with methotrexate (MTX) and CYTARABINE (ara-C) alone or incombination. Material and Methods: MOLT-4 cells were cultured and manitained in RPMI-1640 supplementedwith fetal calf serum, antibiotics and L-gluthamine. Cells were treated with ara-C and MTX with a concentration of 100µmol/L at G1 and 50µmol/L at G2. One and half hour prior to harvesting, cell wereexposed to colcemid. (0.1µg/ml find concentration). Microscopic slides were prepared using standard1000).‚procedure. After staining with 5% Giemsa, metaphase was evaluated using light microscope (×1000). Results: Results indicate that ara-C and MTX, at a 100µmol/L concentration, used for treatment of G1cells, increase the frequency of chromosomal type aberrations; although ara-C was more potent in aberrationinduction. The effect of combined treatment with ara-C and MTX on G1 cells was more than effect of eachdrug when used alone. The effect of these drugs on G2 cells with a concentration of 50µmol/L was found to besignificantly different with that of controls (P<0.05). The frequency of chromatid type breaks induced by ara-C was considerably higher than MTX. When these drug were uaed in combination , the frequencuy of aberration increased dramatically. Conclusion: Results indicate that both ara-C and MTX produce clastogenic effects on MOLT-4 cells.Combination treatment with ara-C and MTX has an additive effect on G1-cells. But a synergistic effect wasfound when they were used for treatment of G2-cells. The reason for hypersensitivity of G2-cells to chemicalsmight be due to shorter repair time of lesions induced in DNA, which are experessed as chromatid typeaberrations in the first mitosis.

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APA: Copy

BARADAR BOKAIE, P.,. (2003). CYTOGENETIC EFFECTS OF METHOTREXATE AND CYTARABINE ALONE AND IN COMBINATION ON G1 AND G2 CELL CYCLE STAGES OF LEUKEMIC MOLT-4 CELL LINE IN VITRO . CELL JOURNAL (YAKHTEH), 5(18), 97-101. SID. https://sid.ir/paper/16252/en

Vancouver: Copy

BARADAR BOKAIE P.. CYTOGENETIC EFFECTS OF METHOTREXATE AND CYTARABINE ALONE AND IN COMBINATION ON G1 AND G2 CELL CYCLE STAGES OF LEUKEMIC MOLT-4 CELL LINE IN VITRO . CELL JOURNAL (YAKHTEH)[Internet]. 2003;5(18):97-101. Available from: https://sid.ir/paper/16252/en

IEEE: Copy

P. BARADAR BOKAIE, an, “CYTOGENETIC EFFECTS OF METHOTREXATE AND CYTARABINE ALONE AND IN COMBINATION ON G1 AND G2 CELL CYCLE STAGES OF LEUKEMIC MOLT-4 CELL LINE IN VITRO ,” CELL JOURNAL (YAKHTEH), vol. 5, no. 18, pp. 97–101, 2003, [Online]. Available: https://sid.ir/paper/16252/en

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