DIFFERENTIATION OF P19 EMBRYONIC CARCINOMA CELLS TO ERYTHROID AND NON ERYTHROID CELLS IN THE PRESENCE OF INTERLEUKIN 3, 6 AND ERYTHROPOIETIN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

YAFTIYAN M.; SALEHNIYA M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2005 | Volume:7 | Issue:25 Page(s): 7-10

Download Full-Text

Persian Verion

View:

868

Download:

Cites:

Information Journal Paper

Title

DIFFERENTIATION OF P19 EMBRYONIC CARCINOMA CELLS TO ERYTHROID AND NON ERYTHROID CELLS IN THE PRESENCE OF INTERLEUKIN 3, 6 AND ERYTHROPOIETIN

Author(s)

YAFTIYAN M. | SALEHNIYA M. | Issue Writer Certificate

Keywords

EMBRYONIC CARCINOMA CELLSQ2

INTERLEUKINQ2

ERYTHROPOIETINQ2

Abstract

Introduction: Setting up a system to study the in vitro hematopoiesis is helpful to understand the mechanism of hematopoiesis. The effects of several cytokines and hormones such as ERYTHROPOIETIN (EPO) in differentiation of hematopoietic stem cells have been investigated in vivo. There are no records considering the potential differentiation of P19 cell line (EMBRYONIC CARCINOMA CELLS) to hematopoietic cells. The aim of this study was to use this cell line as a model to evaluate the effects of INTERLEUKIN 3, 6 and ERYTHROPOIETIN on the differentiation of stem cells.Material and Methods: P19 cells were cultured directly on semisolid medium supplemented with 10% fetal calf serum (FCS). The embryoid bodies were formed 9 days after culturing. The cells were trypsinized and dissociated to single cells. These cells were cultured on the semisolid medium containing 10% FCS, 10 ng/ml IL-3 and IL-6 and in the presence and absence of 20 u/ml ERYTHROPOIETIN. The colonies were formed after 14 days. Then the colonies were studied by benzidine and giemsa staining.Results: Our results showed that in the presence of IL-3, IL-6 and EPO the positive and negative benzidine colonies were 54% and 46%, respectively. In the absence of EPO the mentioned colonies were 14% and 86%, respectively. There was a statistically significant difference between the positive and negative colonies in both groups (p<0.001).Conclusion: Under the effects of IL-3, IL-6 and EPO, the P19 cells could differentiate to erythroid and non-erythroid lineage and in the presences of EPO the umber of erythroid colonies increased.

Cites

No record.

References

No record.

Cite

APA: Copy

YAFTIYAN, M., & SALEHNIYA, M.. (2005). DIFFERENTIATION OF P19 EMBRYONIC CARCINOMA CELLS TO ERYTHROID AND NON ERYTHROID CELLS IN THE PRESENCE OF INTERLEUKIN 3, 6 AND ERYTHROPOIETIN . CELL JOURNAL (YAKHTEH), 7(25), 7-10. SID. https://sid.ir/paper/16268/en

Vancouver: Copy

YAFTIYAN M., SALEHNIYA M.. DIFFERENTIATION OF P19 EMBRYONIC CARCINOMA CELLS TO ERYTHROID AND NON ERYTHROID CELLS IN THE PRESENCE OF INTERLEUKIN 3, 6 AND ERYTHROPOIETIN . CELL JOURNAL (YAKHTEH)[Internet]. 2005;7(25):7-10. Available from: https://sid.ir/paper/16268/en

IEEE: Copy

M. YAFTIYAN, and M. SALEHNIYA, “DIFFERENTIATION OF P19 EMBRYONIC CARCINOMA CELLS TO ERYTHROID AND NON ERYTHROID CELLS IN THE PRESENCE OF INTERLEUKIN 3, 6 AND ERYTHROPOIETIN ,” CELL JOURNAL (YAKHTEH), vol. 7, no. 25, pp. 7–10, 2005, [Online]. Available: https://sid.ir/paper/16268/en

Related Journal Papers