EFFICIENCY OF SIL-SELECT AND PERCOLL TO RECOVER SPERM WITH NORMAL CHROMATIN AND MORPHOLOGY AND EFFECT OF THESE PARAMETERS ON FERTILIZATION, EMBRYO QUALITY AND CLEAVAGE SCORE

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TOFIGH HESABEE S.; NASRESFAHANI MOHAMMAD HOSSEIN; RAZAVI S.H.; MARDANI M.

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Journal: Cell Journal (Yakhteh) Year:2002 | Volume:4 | Issue:15 Page(s): 133-139

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Title

EFFICIENCY OF SIL-SELECT AND PERCOLL TO RECOVER SPERM WITH NORMAL CHROMATIN AND MORPHOLOGY AND EFFECT OF THESE PARAMETERS ON FERTILIZATION, EMBRYO QUALITY AND CLEAVAGE SCORE

Author(s)

TOFIGH HESABEE S. | NASRESFAHANI MOHAMMAD HOSSEIN | RAZAVI S.H. | MARDANI M. | Issue Writer Certificate

Keywords

CHROMATINQ3

SPERM MORPHOLOGYQ4

PERCOLLQ3

SIL-SELECTQ3

CMA3Q3

ANILINE BLUEQ3

Abstract

Introduction: To evaluate and compare efficiency of SIL-SELECT with PERCOLL to recover sperm with normal CHROMATIN in morphology and also studyeffect of these parameters on fertilization, embryo quality and cleavagescore. Materials and Methods: Assessment of semen parameters, protamine deficiency (CMA3) and excessive histone (aniline blue staining) were carried out before and after sperm processing on 46 semen samples from IVF patients referring to Isfahan fertility and infertility center. Results: Both SIL-SELECT and PERCOLL were efficient in elimination of sperms with CHROMATIN and morphologic anomalies. Significant correlatis were obtained between SPERM MORPHOLOGY (strict criteria), protamine deficiencyand excessive histone with in vitro fertilization rate. Among these parameters only SPERM MORPHOLOGY showed a significant correlation with embryo quality and cleavage score. Conclusion: SIL-SELECT is a suitable alternative for PERCOLL to recover sperm with normal CHROMATIN and morphology. Furthermore density gradient techniques are recommended for sperm processing for IVF and especially for ICSI.

Cites

EFFECTS OF SPERMIOGENIC DEFECTS ON FERTILIZATION AND PREGNANCY RATE IN IVF PATIENTS

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APA: Copy

TOFIGH HESABEE, S., NASRESFAHANI, MOHAMMAD HOSSEIN, RAZAVI, S.H., & MARDANI, M.. (2002). EFFICIENCY OF SIL-SELECT AND PERCOLL TO RECOVER SPERM WITH NORMAL CHROMATIN AND MORPHOLOGY AND EFFECT OF THESE PARAMETERS ON FERTILIZATION, EMBRYO QUALITY AND CLEAVAGE SCORE. CELL JOURNAL (YAKHTEH), 4(15), 133-139. SID. https://sid.ir/paper/16330/en

Vancouver: Copy

TOFIGH HESABEE S., NASRESFAHANI MOHAMMAD HOSSEIN, RAZAVI S.H., MARDANI M.. EFFICIENCY OF SIL-SELECT AND PERCOLL TO RECOVER SPERM WITH NORMAL CHROMATIN AND MORPHOLOGY AND EFFECT OF THESE PARAMETERS ON FERTILIZATION, EMBRYO QUALITY AND CLEAVAGE SCORE. CELL JOURNAL (YAKHTEH)[Internet]. 2002;4(15):133-139. Available from: https://sid.ir/paper/16330/en

IEEE: Copy

S. TOFIGH HESABEE, MOHAMMAD HOSSEIN NASRESFAHANI, S.H. RAZAVI, and M. MARDANI, “EFFICIENCY OF SIL-SELECT AND PERCOLL TO RECOVER SPERM WITH NORMAL CHROMATIN AND MORPHOLOGY AND EFFECT OF THESE PARAMETERS ON FERTILIZATION, EMBRYO QUALITY AND CLEAVAGE SCORE,” CELL JOURNAL (YAKHTEH), vol. 4, no. 15, pp. 133–139, 2002, [Online]. Available: https://sid.ir/paper/16330/en

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