CHOLINERGIC DIFFERENTIATION OF NEURAL PRECURSOR CELLS DERIVED FROM MOUSE EMBRYONIC STEM CELLS

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HOMAYOUNI MOGHADAM F.; ALAEI H.A.; KARBALAEI KH.; TANHAEI S.; FESHARAKI MEHRAFARIN; NASRESFAHANI MOHAMMAD HOSSEIN; BAHARVAND H.

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Journal: Physiology and Pharmacology Year:2007 | Volume:11 | Issue:3 Page(s): 192-198

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Title

CHOLINERGIC DIFFERENTIATION OF NEURAL PRECURSOR CELLS DERIVED FROM MOUSE EMBRYONIC STEM CELLS

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Keywords

EMBRYONIC STEM CELLSQ3

NEURONQ3

CHOLINERGICQ3

DIFFERENTIATIONQ3

MOUSEQ3

Abstract

Introduction: EMBRYONIC STEM CELLS (ESCs) isolated from MOUSE blastocyst are genetically normal pluripotent cells and are able to differentiate to all types of cells. Study of mechanism of DIFFERENTIATION of these cells to neural cells could help us to know the mechanism of central nervous system development and finding better strategies for stem cell based therapies in human neurodegenerative disorders. The aim of this study was to evaluate the effect of Sonic hedgehog (Sbh), Retinoic Acid (RA), Leukemia inhibitory factor (LIF), Interleukin-6 (IL6) and nerve growth factor (NGF) on DIFFERENTIATION of neural progenitor cells (NPCs), produced by lineage selection method from MOUSE ESCs, to CHOLINERGIC NEURONs.Methods: Royan BI, MOUSE ESCs derived from C57BL/6 strain was used to produce aggregates. According to growth factor mediated lineage selection method aggregates were cultured in serum free medium to produce nestin positive or NPCs, then cell expansion was achieved by treatment with EGF and FGF2. Following withdrawal of EGF and FGF2, the cells were further cultured in presence or absence of DIFFERENTIATION factors: LIF, Sbh, RA, NGF and IL6 in serum containing medium. Relative number of NEURONs and CHOLINERGIC NEURONs were assessed by immunocytochemical procedure using antibodies against MAP2, b-tubulin 3, and ChAT.Results: Data obtained show that around 70% of the cells were b-tubulin 3 positive. We found different percentage of CHOLINERGIC NEURONs from 10-20% in cultured cells in different groups. RT-PCR analysis showed that CHOLINERGIC specific genes were also expressed in cultured cells.Conclusion: This study shows that some of the NEURONs produced by lineage selection method are CHOLINERGIC NEURONs and the percentage of CHOLINERGIC NEURONs increases after treatment by Sbh, LIF and RA.

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APA: Copy

HOMAYOUNI MOGHADAM, F., ALAEI, H.A., KARBALAEI, KH., TANHAEI, S., FESHARAKI, MEHRAFARIN, NASRESFAHANI, MOHAMMAD HOSSEIN, & BAHARVAND, H.. (2007). CHOLINERGIC DIFFERENTIATION OF NEURAL PRECURSOR CELLS DERIVED FROM MOUSE EMBRYONIC STEM CELLS. PHYSIOLOGY AND PHARMACOLOGY, 11(3), 192-198. SID. https://sid.ir/paper/74953/en

Vancouver: Copy

HOMAYOUNI MOGHADAM F., ALAEI H.A., KARBALAEI KH., TANHAEI S., FESHARAKI MEHRAFARIN, NASRESFAHANI MOHAMMAD HOSSEIN, BAHARVAND H.. CHOLINERGIC DIFFERENTIATION OF NEURAL PRECURSOR CELLS DERIVED FROM MOUSE EMBRYONIC STEM CELLS. PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2007;11(3):192-198. Available from: https://sid.ir/paper/74953/en

IEEE: Copy

F. HOMAYOUNI MOGHADAM, H.A. ALAEI, KH. KARBALAEI, S. TANHAEI, MEHRAFARIN FESHARAKI, MOHAMMAD HOSSEIN NASRESFAHANI, and H. BAHARVAND, “CHOLINERGIC DIFFERENTIATION OF NEURAL PRECURSOR CELLS DERIVED FROM MOUSE EMBRYONIC STEM CELLS,” PHYSIOLOGY AND PHARMACOLOGY, vol. 11, no. 3, pp. 192–198, 2007, [Online]. Available: https://sid.ir/paper/74953/en

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