GRAFT EFFICIENCY OF CO-CULTURED SPERMATOGONIAL CELLS USING SPERM ASSAY IN EPIDIDYMAL LUMEN OF RECIPIENT MICE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ANJAM ROUZ S.H.; MOVAHEDIN M.; TIRAIHI T.; MOULA SEYED JAVAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2006 | Volume:7 | Issue:4 (28) Page(s): 242-249

Download Full-Text

Persian Verion

View:

1,020

Download:

267

Cites:

Information Journal Paper

Title

GRAFT EFFICIENCY OF CO-CULTURED SPERMATOGONIAL CELLS USING SPERM ASSAY IN EPIDIDYMAL LUMEN OF RECIPIENT MICE

Author(s)

ANJAM ROUZ S.H. | MOVAHEDIN M. | TIRAIHI T. | MOULA SEYED JAVAD | Issue Writer Certificate

Keywords

GRAFTQ2

SPERMATOGONIAL CELLSQ2

SPERMATOGENESISQ2

SPERM ASSAYQ2

Abstract

Introduction: Transplantation of germ cells restores the male fertility. Nevertheless, a lot of questions remain incompletely resolved. The aim of this study was to evaluate in vitro colonization efficiency of germ cells and sperm production capacity of SPERMATOGONIAL CELLS before and after culture by sperm number assay in epididymis of recipient mice. Materials and Methods: We developed a Sertoli cell feeder in a co-culture system with SPERMATOGONIAL CELLS and the cells were co-cultured for 2 months. The cells were isolated from mouse neonates. Colony assay was performed during culture using light microscopy. The transplanted cells were traced using BrdU incorporation. Sperm parameters were assessed 2 months after transplantation. Results: Our findings showed that SPERMATOGONIAL CELLS created colonies during culture. Transplantation of fresh SPERMATOGONIAL CELLS at a concentration of 2×105 cells/ml did not show significant differences. However, after transplantation of 2×105 cells/ml cultured for 2 weeks, the number of epididymal sperms in recipients increased significantly in groups with more fresh cells. Conclusion: Epididymal sperm number in recipient mice can be increased by enrichment of type A SPERMATOGONIAL CELLS using an in vitro co-culture system. Other important factors include the source of donor cells and the number of transplanted cells.

Cites

No record.

References

Cite

APA: Copy

ANJAM ROUZ, S.H., MOVAHEDIN, M., TIRAIHI, T., & MOULA, SEYED JAVAD. (2006). GRAFT EFFICIENCY OF CO-CULTURED SPERMATOGONIAL CELLS USING SPERM ASSAY IN EPIDIDYMAL LUMEN OF RECIPIENT MICE. CELL JOURNAL (YAKHTEH), 7(4 (28)), 242-249. SID. https://sid.ir/paper/16370/en

Vancouver: Copy

ANJAM ROUZ S.H., MOVAHEDIN M., TIRAIHI T., MOULA SEYED JAVAD. GRAFT EFFICIENCY OF CO-CULTURED SPERMATOGONIAL CELLS USING SPERM ASSAY IN EPIDIDYMAL LUMEN OF RECIPIENT MICE. CELL JOURNAL (YAKHTEH)[Internet]. 2006;7(4 (28)):242-249. Available from: https://sid.ir/paper/16370/en

IEEE: Copy

S.H. ANJAM ROUZ, M. MOVAHEDIN, T. TIRAIHI, and SEYED JAVAD MOULA, “GRAFT EFFICIENCY OF CO-CULTURED SPERMATOGONIAL CELLS USING SPERM ASSAY IN EPIDIDYMAL LUMEN OF RECIPIENT MICE,” CELL JOURNAL (YAKHTEH), vol. 7, no. 4 (28), pp. 242–249, 2006, [Online]. Available: https://sid.ir/paper/16370/en

Related Journal Papers