METHYLATION STATUS OF P15 GENE IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA USING METHYLATION-SPECIFIC PCR

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NAJAR SADEGHI R.A.; MESBAH NAMIN S.A.R.; RASTGAR JAZI F.

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Journal: Cell Journal (Yakhteh) Year:2007 | Volume:9 | Issue:1 (33) Page(s): 39-44

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Information Journal Paper

Title

METHYLATION STATUS OF P15 GENE IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA USING METHYLATION-SPECIFIC PCR

Author(s)

NAJAR SADEGHI R.A. | MESBAH NAMIN S.A.R. | RASTGAR JAZI F. | Issue Writer Certificate

Keywords

ESOPHAGEAL SQUAMOUS CELL CARCINOMAQ2

P15Q2

DNA METHYLATIONQ1

METHYLATION SPECIFIC PCRQ2

Abstract

Introduction: Promoter methylation has an inhibitory effect on gene expression. Thus we investigated methylation patterns of P15 gene in ESOPHAGEAL SQUAMOUS CELL CARCINOMA (ESCC), one of the most frequent and fetal cancers in the world. Materials and Methods: P15 promoter methylation status of 30 ESCC and 10 normal specimens was investigated using METHYLATION SPECIFIC PCR. DNA extracted from the specimens was digested by restriction enzyme. The digested DNA was modified by sodium bisulfite. Using methylated and unmethylated specific primers, methylation status was determined based on the presence of the methylated and unmethylated specific PCR products. Also DNA extracted from normal blood and CpG methylase (SssI)–treated genomic DNA were used as the negative and positive controls respectively.Results: Our results showed that all normal specimens were unmethyalted and only 5 out of 30 tumor samples (16.6%) were methylated. Statistical analysis (Fisher’s exact tests) showed no significant correlation between P15 gene promoter methylation and ESCC cancer.Conclusion: According to the results of the study, P15 gene does not play a significant role in aberrant methylation process in ESCC, other mechanisms may involve in P15 inactivation during ESCC development. However, due to limited number of specimens, we recommend further investigation using a larger sample.

Cites

GLOBAL GENOME METHYLATION STATUS IN GASTRITIS LESION IN COMPARISON WITH NORMAL ADJACENT TISSUE AND ITS RELATIONSHIP WITH CLINICOPATHOLOGIC FINDINGS

References

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APA: Copy

NAJAR SADEGHI, R.A., MESBAH NAMIN, S.A.R., & RASTGAR JAZI, F.. (2007). METHYLATION STATUS OF P15 GENE IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA USING METHYLATION-SPECIFIC PCR. CELL JOURNAL (YAKHTEH), 9(1 (33)), 39-44. SID. https://sid.ir/paper/16396/en

Vancouver: Copy

NAJAR SADEGHI R.A., MESBAH NAMIN S.A.R., RASTGAR JAZI F.. METHYLATION STATUS OF P15 GENE IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA USING METHYLATION-SPECIFIC PCR. CELL JOURNAL (YAKHTEH)[Internet]. 2007;9(1 (33)):39-44. Available from: https://sid.ir/paper/16396/en

IEEE: Copy

R.A. NAJAR SADEGHI, S.A.R. MESBAH NAMIN, and F. RASTGAR JAZI, “METHYLATION STATUS OF P15 GENE IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA USING METHYLATION-SPECIFIC PCR,” CELL JOURNAL (YAKHTEH), vol. 9, no. 1 (33), pp. 39–44, 2007, [Online]. Available: https://sid.ir/paper/16396/en

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