INVESTIGATION OF THE ABERRANT DNA METHYLATION OF P14, P15, P16 GENES IN PATIENTS WITH ESCC IN IRAN

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MOHAMMAD GANJI SH.; MAHBOUDI S.; RASTGAR JAZII F.

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Journal: Journal of Molecular and Cellular Researches Year:2015 | Volume:28 | Issue:3 Page(s): 403-412

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Title

INVESTIGATION OF THE ABERRANT DNA METHYLATION OF P14, P15, P16 GENES IN PATIENTS WITH ESCC IN IRAN

Author(s)

MOHAMMAD GANJI SH. | MAHBOUDI S. | RASTGAR JAZII F. | Issue Writer Certificate

Keywords

METHYLATIONQ2

P14Q2

P15Q2

P16Q2

ESOPHAGEAL SQUAMOUS CELL CARCINOMA(ESCC)Q2

Abstract

Studies have shown that aberrant METHYLATION of the tumor suppressor genes could be lead to silencing and reducing of their gene expression, consequently occurring the cancer.The aim of this study was investigation of the aberrantDNAMETHYLATION of P14, P15, P16 genes in Iranian patients with Esophageal Squamous cell carcinoma (ESCC) and its association with demographic and pathological characteristics. Therefore we extracted the DNAs and RNAs of44 tumor tissues and 19 normal tissues from 44 unrelated Iranian patients with ESCC. The METHYLATION specific PCR (MSPCR) was performed for both methylated and unmethylated status of these genes and the expression of these genes have done with RT-PCR. The results showed the 53%, 9% and 27% METHYLATION for the P14, P15 and P16 genes respectively in the tumor tissues and no METHYLATION frequency in none of the normal sample. Regarding the gene expression, there was an inverse relationship between METHYLATION status and gene expression (p<0.05). The frequency of METHYLATION of P15 and P14 in tumor samples was 41%, 9% respectively and abundance of METHYLATION the P15+p16 and P14+p15, was 6/13%, and 5/4%, respectively, while the frequency of METHYLATION in P14+p16 genes was observed 9% (P=0.0036). It means that all patients which are methylated for the P14 had the METHYLATION in P16 too while in most patients with P15 methylated, the P14 or P16 METHYLATION was not found. Comparing these results with other reports shows a similarity between the Iranian population and Chinese population (North of China) maybe because of a similar molecular mechanisms and etiology of ESCC cancer in these populations.

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APA: Copy

MOHAMMAD GANJI, SH., MAHBOUDI, S., & RASTGAR JAZII, F.. (2015). INVESTIGATION OF THE ABERRANT DNA METHYLATION OF P14, P15, P16 GENES IN PATIENTS WITH ESCC IN IRAN. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(3), 403-412. SID. https://sid.ir/paper/248462/en

Vancouver: Copy

MOHAMMAD GANJI SH., MAHBOUDI S., RASTGAR JAZII F.. INVESTIGATION OF THE ABERRANT DNA METHYLATION OF P14, P15, P16 GENES IN PATIENTS WITH ESCC IN IRAN. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(3):403-412. Available from: https://sid.ir/paper/248462/en

IEEE: Copy

SH. MOHAMMAD GANJI, S. MAHBOUDI, and F. RASTGAR JAZII, “INVESTIGATION OF THE ABERRANT DNA METHYLATION OF P14, P15, P16 GENES IN PATIENTS WITH ESCC IN IRAN,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 3, pp. 403–412, 2015, [Online]. Available: https://sid.ir/paper/248462/en

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