ASSESSMENT OF FERTILIZATION AND DEVELOPMENT OF NMRI FETUS USING DIFFERENT CULTURE MEDIA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHAHVERDI A.A.H.; MOVAHEDIN MANSOUREH; REZAZADEH VALOUJERDI M.; KAZEMI ASHTIANI S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2007 | Volume:8 | Issue:4 (32) Page(s): 232-237

Download Full-Text

Persian Verion

View:

1,042

Download:

Cites:

Information Journal Paper

Title

ASSESSMENT OF FERTILIZATION AND DEVELOPMENT OF NMRI FETUS USING DIFFERENT CULTURE MEDIA

Author(s)

SHAHVERDI A.A.H. | MOVAHEDIN MANSOUREH | REZAZADEH VALOUJERDI M. | KAZEMI ASHTIANI S. | Issue Writer Certificate

Keywords

MOUSE EMBRYOQ4

DEVELOPMENTQ2

CO CULTUREQ2

Abstract

Introduction: Determination of best culture medium for fertilization and DEVELOPMENT of NMRI mice.Material and Methods: DEVELOPMENT of fertilized mature oocytes of NMRI mice was studied for five days in several culture media (KSOM, T6, M16, CZB, G1TM ver3 and G2TM ver3) following hormone therapy with PMSG and HCG.Results: The rate of fertilization after 24 hours was 97.4% in KSOM, 83.5% in T6, 90.5% in M16, 100% in CZB and 90.9% in G1TM ver3 and G2TM ver3. The differences between CZB and KSOM culture media in comparison to T6 and M16 and G1TM ver3 and G2TM ver3 media were statistically significant (p<0.05). Blastocyte stage was seen in 96.3% of CZB group which was the highest in comparison to other culture media (p£0.05). Blastocyte formation was 80.2% in T6, 82.1% in M16, 77.6% in KSOM and 87.5% in G1TM ver3 and G2TM ver3 culture media. The highest Hatching blastocyst rate was seen in sequential medium (G1TM ver3 and G2TM ver3) and KSOM culture medium (76.1% and 66.4%, respectively) while the lowest rate was 60.3% in M16 medium. The rate of embryo degeneration was lowest in sequential medium (G1TM ver3 and G2TM ver3) (23.9%) in comparison to other groups and the difference was statistically significant regarding T6 group (51.6%) (p<0.01)Conclusion: This study reveals that CZB, KSOM and G1TM ver3 and G2TM ver3 are the best culture media for pre implantation embryo DEVELOPMENT of NMRI mice. The difference in DEVELOPMENT of mice embryos in different culture media can be due to difference in medium supplements (aminoacids) and concentration of culture medium components.

Cites

No record.

References

No record.

Cite

APA: Copy

SHAHVERDI, A.A.H., MOVAHEDIN, MANSOUREH, REZAZADEH VALOUJERDI, M., & KAZEMI ASHTIANI, S.. (2007). ASSESSMENT OF FERTILIZATION AND DEVELOPMENT OF NMRI FETUS USING DIFFERENT CULTURE MEDIA. CELL JOURNAL (YAKHTEH), 8(4 (32)), 232-237. SID. https://sid.ir/paper/16530/en

Vancouver: Copy

SHAHVERDI A.A.H., MOVAHEDIN MANSOUREH, REZAZADEH VALOUJERDI M., KAZEMI ASHTIANI S.. ASSESSMENT OF FERTILIZATION AND DEVELOPMENT OF NMRI FETUS USING DIFFERENT CULTURE MEDIA. CELL JOURNAL (YAKHTEH)[Internet]. 2007;8(4 (32)):232-237. Available from: https://sid.ir/paper/16530/en

IEEE: Copy

A.A.H. SHAHVERDI, MANSOUREH MOVAHEDIN, M. REZAZADEH VALOUJERDI, and S. KAZEMI ASHTIANI, “ASSESSMENT OF FERTILIZATION AND DEVELOPMENT OF NMRI FETUS USING DIFFERENT CULTURE MEDIA,” CELL JOURNAL (YAKHTEH), vol. 8, no. 4 (32), pp. 232–237, 2007, [Online]. Available: https://sid.ir/paper/16530/en

Related Journal Papers