THE EVALUATION OF MELATONIN EFFECT ON IN-VITRO DEVELOPMENT OF MOUSE PREIMPLANTATION EMBRYOS

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ASGARI Z.; RAMEZANI M.; BAHADORI M.H.; AHMADI JALALI MOGHADDAM M.

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Journal: Anatomical Sciences Journal Year:2011 | Volume:8 | Issue:32-33 Page(s): 164-168

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Title

THE EVALUATION OF MELATONIN EFFECT ON IN-VITRO DEVELOPMENT OF MOUSE PREIMPLANTATION EMBRYOS

Author(s)

ASGARI Z. | RAMEZANI M. | BAHADORI M.H. | AHMADI JALALI MOGHADDAM M. | Issue Writer Certificate

Keywords

MELATONINQ2

PREIMPLANTATION EMBRYOQ3

DEVELOPMENTQ3

MOUSEQ3

Abstract

Abstract Purpose: MELATONIN promotes in-vitro embryo DEVELOPMENT in different species. This study studied the effects of MELATONIN on in-vitro MOUSE PREIMPLANTATION EMBRYO DEVELOPMENT.Materials and Methods: Two-cell embryos were obtained from oviduct of 6-8 weeks female NMRI mice 48 hours after administration of an intra-peritoneal injection of 5 IU/ml pregnant mares’ serum gonadotrophin and subsequent 5 IU/ml human chorionic gonadotrophin (ip). Embryos were cultured in T6 culture medium supplemented with different dosages of MELATONIN {0 (control group), 100×10-6 M, 10×10-6 M, 1×10-6 M, 100×10-9 M and 10×10-9 M (1-5 treatment groups)}. With due attention to percent of embryos in different stages, the rate of DEVELOPMENT of embryos was assessed using of invert microscope. It is also compared to control group.Results: The results showed that the rate of cleavage and DEVELOPMENT of MOUSE the embryos to blastocyst stage increased significantly in the DEVELOPMENT culture medium supplemented with 10 and 100 nM/mg of MELATONIN in comparison to control group (p<0.001).Conclusion: The results of this study demonstrate that, enriching the culture medium with MELATONIN, improve MOUSE preimplantation DEVELOPMENT.

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APA: Copy

ASGARI, Z., RAMEZANI, M., BAHADORI, M.H., & AHMADI JALALI MOGHADDAM, M.. (2011). THE EVALUATION OF MELATONIN EFFECT ON IN-VITRO DEVELOPMENT OF MOUSE PREIMPLANTATION EMBRYOS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 8(32-33), 164-168. SID. https://sid.ir/paper/81430/en

Vancouver: Copy

ASGARI Z., RAMEZANI M., BAHADORI M.H., AHMADI JALALI MOGHADDAM M.. THE EVALUATION OF MELATONIN EFFECT ON IN-VITRO DEVELOPMENT OF MOUSE PREIMPLANTATION EMBRYOS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2011;8(32-33):164-168. Available from: https://sid.ir/paper/81430/en

IEEE: Copy

Z. ASGARI, M. RAMEZANI, M.H. BAHADORI, and M. AHMADI JALALI MOGHADDAM, “THE EVALUATION OF MELATONIN EFFECT ON IN-VITRO DEVELOPMENT OF MOUSE PREIMPLANTATION EMBRYOS,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 8, no. 32-33, pp. 164–168, 2011, [Online]. Available: https://sid.ir/paper/81430/en

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