PURIFICATION AND STUDY OF PROTEOLYTIC ACTIVITY OF FICIN ENZYME OF FIG (FICUS CARICA)

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ZHALEHJOO N.; CHALABI M.; PARVANEH SH.; MOSTAFAIE A.

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Journal: Avicenna Journal of Clinical Medicine Year:2013 | Volume:20 | Issue:2 (SN 68) Page(s): 126-132

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Title

PURIFICATION AND STUDY OF PROTEOLYTIC ACTIVITY OF FICIN ENZYME OF FIG (FICUS CARICA)

Author(s)

ZHALEHJOO N. | CHALABI M. | PARVANEH SH. | MOSTAFAIE A. | Issue Writer Certificate

Keywords

FICAINQ2

FIGQ2

PROTEASESQ2

Abstract

Introduction & Objective: Ficin is a member of plant cystein PROTEASES that is abundant in FIG.This enzyme has many pharmacological and industrial uses. In the present study, the enzyme was purified by a simple procedure and its proteolytic activity was assayed on several plant and animal proteins.Materials & Methods: Ficin was extracted from unripe FIG, precipitated by ammonium sulfate and purified using ion-exchange chromatography on a Carboxymethyl Sepharose column.Proteolytic activities of the purified enzyme were determined in 4 buffering conditions on casein, alpha lactalbumin, beta lactoglobulin and gelatin proteins.Results: Purified enzymes include two bands with molecular mass of 24 and 26 KDa. Results of proteolytic activity showed that ficin can digest casein. It has moderate hydrolytic activity on beta lactoglobulin and gelatin but ficin can not hydrolyze alpha lactalbumin.Conclusion: It seems ficin has selective effects on some proteins so it can be a good candidate for digestion of casein and making related drugs.

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APA: Copy

ZHALEHJOO, N., CHALABI, M., PARVANEH, SH., & MOSTAFAIE, A.. (2013). PURIFICATION AND STUDY OF PROTEOLYTIC ACTIVITY OF FICIN ENZYME OF FIG (FICUS CARICA). AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 20(2 (SN 68)), 126-132. SID. https://sid.ir/paper/17485/en

Vancouver: Copy

ZHALEHJOO N., CHALABI M., PARVANEH SH., MOSTAFAIE A.. PURIFICATION AND STUDY OF PROTEOLYTIC ACTIVITY OF FICIN ENZYME OF FIG (FICUS CARICA). AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2013;20(2 (SN 68)):126-132. Available from: https://sid.ir/paper/17485/en

IEEE: Copy

N. ZHALEHJOO, M. CHALABI, SH. PARVANEH, and A. MOSTAFAIE, “PURIFICATION AND STUDY OF PROTEOLYTIC ACTIVITY OF FICIN ENZYME OF FIG (FICUS CARICA),” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 20, no. 2 (SN 68), pp. 126–132, 2013, [Online]. Available: https://sid.ir/paper/17485/en

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