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Information Journal Paper

Title

EVALUATION OF AFLATOXIN B1 REDUCTION IN PRESENT OF LACTOBACILLI ISOLATED FROM TARKHINEH AND FERMENTED MILK OF TARKHINEH BY ELISA

Pages

  43-48

Abstract

 Background and Objective: AFLATOXINs are the most important mycotoxin that produced by fungi. Some strains of LAB can control mutagens factors like AFLATOXIN. It seems that it is because of physical attachment between LAB and mutagens. The aim of this study is evaluation ability of 32LACTOBACILLI strains isolated from TARKHINEH to attach and remove AFLATOXIN from buffer solution by ELISA.Materials and Methods: An overnight culture of each LACTOBACILLI (1010 CFU/ml) was transferred into PBS buffer with 5mg/ml AFLATOXIN. After 2h incubation cells were removed by centrifuge and remained AFLATOXIN was assessed by ELISA. In order to control all physicochemical factors temperature were kept in 37oC in neutralized condition.Results: The strains were observed to possess variable AFB2-binding ability in the range from 8.4 to 89.9%. Most efficient binding of AFB1 was observed by isolatesTD2, TD14, and TD15 while T1 and T5 showed the least capacity.Conclusion: Our results indicated that Lactobacillus strains isolated from TARKHINEH could sequestrate of AFLATOXIN by binding the toxin in a strain dependent manner. So After more safety assessment these isolates can be used in food industry as a native probiotic.

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    APA: Copy

    TAJABADI EBRAHIMI, M., JAFARI, P., BAHRAMI, H., & HASHEMI, M.. (2011). EVALUATION OF AFLATOXIN B1 REDUCTION IN PRESENT OF LACTOBACILLI ISOLATED FROM TARKHINEH AND FERMENTED MILK OF TARKHINEH BY ELISA. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 3(8), 43-48. SID. https://sid.ir/paper/181437/en

    Vancouver: Copy

    TAJABADI EBRAHIMI M., JAFARI P., BAHRAMI H., HASHEMI M.. EVALUATION OF AFLATOXIN B1 REDUCTION IN PRESENT OF LACTOBACILLI ISOLATED FROM TARKHINEH AND FERMENTED MILK OF TARKHINEH BY ELISA. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2011;3(8):43-48. Available from: https://sid.ir/paper/181437/en

    IEEE: Copy

    M. TAJABADI EBRAHIMI, P. JAFARI, H. BAHRAMI, and M. HASHEMI, “EVALUATION OF AFLATOXIN B1 REDUCTION IN PRESENT OF LACTOBACILLI ISOLATED FROM TARKHINEH AND FERMENTED MILK OF TARKHINEH BY ELISA,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 3, no. 8, pp. 43–48, 2011, [Online]. Available: https://sid.ir/paper/181437/en

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