MOLECULAR IDENTIFICATION OF PROBIOTIC LACTOBACILL BACTERIA FROM TARKHINEH AND TARKHINEH DOUGH BY 16S RDNA SEQUENCING

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TAFVIZI F.; TAJABADI EBRAHIMI M.; KHOJAREH L.

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Journal: Journal of Food Technology and Nutrition Year:2013 | Volume:10 | Issue:2 (38) Page(s): 61-68

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Information Journal Paper

Title

MOLECULAR IDENTIFICATION OF PROBIOTIC LACTOBACILL BACTERIA FROM TARKHINEH AND TARKHINEH DOUGH BY 16S RDNA SEQUENCING

Author(s)

TAFVIZI F. | TAJABADI EBRAHIMI M. | KHOJAREH L. | Issue Writer Certificate

Keywords

LACTOBACILUSQ3

SEQUENCINGQ3

TARKHINEHQ3

16S RRNAQ3

Abstract

Introduction: TARKHINEH soup is one of the traditional foods consumed in the west part of Iran due to its medical and pharmaceutical properties. Studies have indicated that TARKHINEH is a good source of probiotic bacteria. Therefore molecular identification of lactobacillus strains of TARKHINEH by 16S RRNA gene SEQUENCING might be considered essential.Materials and Methods: 4 isolates (T20, T22, TD4, TD10) were selected for molecular identification. Samples were grown in MRS broth and total genomic DNA was extracted by lysosyme extraction protocol. PCR-mediated amplification of the 16 S rDNA was carried out by specific primers. Identification of bacterial isolates was performed based on 16s rDNA partial gene sequence method for both strands with the same primer used in PCR.Results: These strains produced the high level of bacteriocin.16S rRNA genes were sequenced and deposited as a novel stains in Gen Bank. The sequences were compared with other rRNA sequences available in the NCBI database and the high similarity (99%) to speciesL. casei was estimated for the sequence isolates.Conclusion: Due to the high potential of probiotic bacteria in TARKHINEH, these strains might be considered useful and could be employed in food industry.

Cites

REPAIR ETHANOL INDUCED AMNESIA WITH IRANIAN LACTOBACILLUS BREVIS IN RATS

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APA: Copy

TAFVIZI, F., TAJABADI EBRAHIMI, M., & KHOJAREH, L.. (2013). MOLECULAR IDENTIFICATION OF PROBIOTIC LACTOBACILL BACTERIA FROM TARKHINEH AND TARKHINEH DOUGH BY 16S RDNA SEQUENCING. JOURNAL OF FOOD TECHNOLOGY AND NUTRITION, 10(2 (38)), 61-68. SID. https://sid.ir/paper/143417/en

Vancouver: Copy

TAFVIZI F., TAJABADI EBRAHIMI M., KHOJAREH L.. MOLECULAR IDENTIFICATION OF PROBIOTIC LACTOBACILL BACTERIA FROM TARKHINEH AND TARKHINEH DOUGH BY 16S RDNA SEQUENCING. JOURNAL OF FOOD TECHNOLOGY AND NUTRITION[Internet]. 2013;10(2 (38)):61-68. Available from: https://sid.ir/paper/143417/en

IEEE: Copy

F. TAFVIZI, M. TAJABADI EBRAHIMI, and L. KHOJAREH, “MOLECULAR IDENTIFICATION OF PROBIOTIC LACTOBACILL BACTERIA FROM TARKHINEH AND TARKHINEH DOUGH BY 16S RDNA SEQUENCING,” JOURNAL OF FOOD TECHNOLOGY AND NUTRITION, vol. 10, no. 2 (38), pp. 61–68, 2013, [Online]. Available: https://sid.ir/paper/143417/en

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