THE PREVALENCE OF ADHESIVE SURFACE ENCODING GENES IN STAPHYLOCOCCUS AUREUS ISOLATED FROM HOSPITALIZED PATIENTS IN ZABOL-IRAN BY MULTIPLEX PCR

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MOLLAEI M.; RASHKI A.

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Journal: JOURNAL OF ADVANCED BIOMEDICAL SCIENCES Year:2016 | Volume:6 | Issue:3 Page(s): 296-302

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Title

THE PREVALENCE OF ADHESIVE SURFACE ENCODING GENES IN STAPHYLOCOCCUS AUREUS ISOLATED FROM HOSPITALIZED PATIENTS IN ZABOL-IRAN BY MULTIPLEX PCR

Author(s)

MOLLAEI M. | RASHKI A. | Issue Writer Certificate

Keywords

STAPHYLOCOCCUS AUREUSQ1

FIBRINOGENQ2

FIBRONECTINSQ2

MULTIPLEX PCRQ2

Abstract

Background & Objective: STAPHYLOCOCCUS AUREUS is one of the most frequently opportunistic pathogens isolated from nosocomial infections, responsible for severe infections such as bacteremia, endocarditis, and skin infections. Surface proteins such as FIBRINOGEN and fibronectin-binding proteins are important factors in adhesion and invasion of S. aureus. Therefore, the objective of this study is to evaluate the presence of genes clfA, clfB, fnbA, and fnbB in isolates of S. aureus collected from clinical specimens of hospitalized patients in Hospitals of Zabol -Iran.Materials & Methods: In this cross-sectional study, 100 S. aureus isolates were collected from January to August 2013 from hospitalized patients at zabol-Iran. The isolates were confirmed by conventional biochemical tests. DNA of all isolates was extracted by boiling method. MULTIPLEX PCR was used to identify the presence of virulence genes. The data were analyzed using Fisher's exact test.Results: The results of this study showed that 50% of isolates possess at least one of the studied genes .The frequency of genes encoding FIBRINOGEN (clfA, clfB) and fibronectin (fnbA, fnbB) were 19%, 16% and 19%, 25% respectively.Conclusion: This study showed that the studied genes are found in small percentage of isolates. Further investigations on these genes are needed to clarify their role in the pathogenesis of S. aureus infections.

Cites

Survey the Role and Effect of Some Antibiotic Resistance in the Spread of Pathogenic Strains of Staphylococcus aureus in Different Clinical Specimens

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APA: Copy

MOLLAEI, M., & RASHKI, A.. (2016). THE PREVALENCE OF ADHESIVE SURFACE ENCODING GENES IN STAPHYLOCOCCUS AUREUS ISOLATED FROM HOSPITALIZED PATIENTS IN ZABOL-IRAN BY MULTIPLEX PCR. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 6(3), 296-302. SID. https://sid.ir/paper/203987/en

Vancouver: Copy

MOLLAEI M., RASHKI A.. THE PREVALENCE OF ADHESIVE SURFACE ENCODING GENES IN STAPHYLOCOCCUS AUREUS ISOLATED FROM HOSPITALIZED PATIENTS IN ZABOL-IRAN BY MULTIPLEX PCR. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2016;6(3):296-302. Available from: https://sid.ir/paper/203987/en

IEEE: Copy

M. MOLLAEI, and A. RASHKI, “THE PREVALENCE OF ADHESIVE SURFACE ENCODING GENES IN STAPHYLOCOCCUS AUREUS ISOLATED FROM HOSPITALIZED PATIENTS IN ZABOL-IRAN BY MULTIPLEX PCR,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 6, no. 3, pp. 296–302, 2016, [Online]. Available: https://sid.ir/paper/203987/en

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