PHENOTYPIC AND GENOTYPIC STUDY OF STAPHYLOCOCCUS AUREUS BIOFILM GENES ISOLATED FROM CLINICAL AND FOOD CASES USING MICROTITRPLATE AND MULTIPLEX PCR

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MADANI SAREH BEIGOM; AMINI KUMARSS

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Journal: Research in Medicine Year:2018 | Volume:42 | Issue:1 Page(s): 52-58

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Title

PHENOTYPIC AND GENOTYPIC STUDY OF STAPHYLOCOCCUS AUREUS BIOFILM GENES ISOLATED FROM CLINICAL AND FOOD CASES USING MICROTITRPLATE AND MULTIPLEX PCR

Author(s)

MADANI SAREH BEIGOM | AMINI KUMARSS | Issue Writer Certificate

Keywords

STAPHYLOCOCCUS AUREUSQ2

BIOFILM GENESQ1

MULTIPLEX PCRQ1

Abstract

Background: The most important factor in the formation of STAPHYLOCOCCUS AUREUS is the ability to form biofilms. This bacterium has the ability to bind and penetrate into tissue cells. Biofilmic infections are usually chronic and reversible and respond to difficult treatments. The purpose of the present study was to identify BIOFILM GENES in STAPHYLOCOCCUS AUREUS isolated from clinical and food samples using multiple PCR methods.Materials and Methods: A total of 60 isolates of STAPHYLOCOCCUS AUREUS, cultured from human and food after extraction of genome, were used to study the presence of adherent genes using multiple PCR techniques. The phenotypic study of biofilm formation was performed using Microtiter plates assay microplate technique.Results: In total, 60 human and food samples were studied that included 6 samples of foodstuff, 20% of samples and of 2 samples clinical 6.67% isolated from biofilms. In 30 isolates isolated from food, the frequencies of fnbB, clfB, and clfA genes were identified as 20%, 76.66%, 80%, respectively. However, among the 30 clinical samples, the frequencies of fnbB, clfB, and clfA genes were 6.66%, 70%, and 93.33%, respectively. The fnbA gene was not detected in any of the samples.Conclusion: The results of the study showed that the high prevalence of biofilm-inducing genes and also a relatively high phenotypic expression among the samples showed that it could be related to the resistance of the isolates of STAPHYLOCOCCUS AUREUS.

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APA: Copy

MADANI, SAREH BEIGOM, & AMINI, KUMARSS. (2018). PHENOTYPIC AND GENOTYPIC STUDY OF STAPHYLOCOCCUS AUREUS BIOFILM GENES ISOLATED FROM CLINICAL AND FOOD CASES USING MICROTITRPLATE AND MULTIPLEX PCR. PAJOUHESH DAR PEZESHKI, 42(1 ), 52-58. SID. https://sid.ir/paper/42220/en

Vancouver: Copy

MADANI SAREH BEIGOM, AMINI KUMARSS. PHENOTYPIC AND GENOTYPIC STUDY OF STAPHYLOCOCCUS AUREUS BIOFILM GENES ISOLATED FROM CLINICAL AND FOOD CASES USING MICROTITRPLATE AND MULTIPLEX PCR. PAJOUHESH DAR PEZESHKI[Internet]. 2018;42(1 ):52-58. Available from: https://sid.ir/paper/42220/en

IEEE: Copy

SAREH BEIGOM MADANI, and KUMARSS AMINI, “PHENOTYPIC AND GENOTYPIC STUDY OF STAPHYLOCOCCUS AUREUS BIOFILM GENES ISOLATED FROM CLINICAL AND FOOD CASES USING MICROTITRPLATE AND MULTIPLEX PCR,” PAJOUHESH DAR PEZESHKI, vol. 42, no. 1 , pp. 52–58, 2018, [Online]. Available: https://sid.ir/paper/42220/en

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