AN ANALYSIS OF MOLECULAR EXPRESSION OF GABAA RECEPTOR SUBUNIT GENES IN HUMAN EMBRYONIC KIDNEY 293 CELLS

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ESMAEILI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2011 | Volume:24 | Issue:2 Page(s): 162-168

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Title

AN ANALYSIS OF MOLECULAR EXPRESSION OF GABAA RECEPTOR SUBUNIT GENES IN HUMAN EMBRYONIC KIDNEY 293 CELLS

Author(s)

ESMAEILI A. | Issue Writer Certificate

Keywords

HEK 293 CELLSQ2

GABAA RECEPTORSQ2

EXPRESSION SYSTEMQ2

RT-PCRQ2

Abstract

HEK (human embryonic kidney) 293 cells as transient transfection systems are the most useful expression method to study physiological and pharmacological properties of the GABAA receptor subunits. HEK properties are similar to the native expressed channels. In comparison to the other common artificial EXPRESSION SYSTEMs, HEK cells have a similar mammalian intracellular environment. Since some mRNAs such as GABAA g3 are significantly found in HEK 293 CELLS, caution must be taken in interpreting the results of studies of the properties of GABAA receptors expressed in HEK 293 CELLS. In this study we examined the endogenous mRNA content of GABAA receptor subunits a1, a2, g1 and g2 in HEK 293 CELLS using traditional PCR and real time PCR. Total cellular RNA was extracted from HEK 293 CELLS and reverse transcribed to cDNA. PCR and real time PCR reactions were performed in a 25 ml volume containing specific forward and reverse primers for GABAAR subunits with other required reagents. Reactions were carried out using a thermocycler. The amplification products were analyzed on 2 % agarose gels stained with ethidium bromide. In this study, none of GABAAR subunits in HEK cells were detected. The results show that HEK 293 cell line can be used as an EXPRESSION SYSTEM for the analysis of GABAA receptor recombinants.

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APA: Copy

ESMAEILI, A.. (2011). AN ANALYSIS OF MOLECULAR EXPRESSION OF GABAA RECEPTOR SUBUNIT GENES IN HUMAN EMBRYONIC KIDNEY 293 CELLS. IRANIAN JOURNAL OF BIOLOGY, 24(2), 162-168. SID. https://sid.ir/paper/21212/en

Vancouver: Copy

ESMAEILI A.. AN ANALYSIS OF MOLECULAR EXPRESSION OF GABAA RECEPTOR SUBUNIT GENES IN HUMAN EMBRYONIC KIDNEY 293 CELLS. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2011;24(2):162-168. Available from: https://sid.ir/paper/21212/en

IEEE: Copy

A. ESMAEILI, “AN ANALYSIS OF MOLECULAR EXPRESSION OF GABAA RECEPTOR SUBUNIT GENES IN HUMAN EMBRYONIC KIDNEY 293 CELLS,” IRANIAN JOURNAL OF BIOLOGY, vol. 24, no. 2, pp. 162–168, 2011, [Online]. Available: https://sid.ir/paper/21212/en

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