SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN CANOLA (BRASSICA NAPUS L.)

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MAJD AHMAD; CHAMAN DOUSTI F.; MEHRABIAN SEDIGHEH; SHEYDAEI MASOUD

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Paper Information

Journal: IRANIAN JOURNAL OF BIOLOGY Year:2008 | Volume:20 | Issue:4 Page(s): 344-352

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Information Journal Paper

Title

SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN CANOLA (BRASSICA NAPUS L.)

Author(s)

MAJD AHMAD | CHAMAN DOUSTI F. | MEHRABIAN SEDIGHEH | SHEYDAEI MASOUD | Issue Writer Certificate

Keywords

SOMATIC EMBRYOSQ3

SODIUM CHLORIDEQ2

CANOLAQ2

Abstract

In this study a simple method for the induction and development of SOMATIC EMBRYOS from CANOLA (Brassica napus L.) hypocotyl explants is determined and reported. Hypocotyl explants were found to be more suitable than other plant parts for somatic embryogenesis. A low concentration of sucrose (20 g/lit) enhanced somatic embryogenesis, but greater concentrations (60 g/lit) decreased embryogenetic potential of the Calli. On modified MS medium containing 2% sucrose, 1 mg/lit 2,4-D, 2 mg/lit NAA and 2 mg/lit BAP each explant developed calli. Different NaCl concentrations (0, 2, 3, 4, 5, 6, 7, and 8 mg/lit) were tested and somatic embryogenesis was improved in the presence of 6 mg/lit NaCl. Embryoids developed shoots on the same medium and rooted after being transported to a medium supplemented with 1 mg/lit IBA. Regenerated plants were transfered to pots after adaptation to dry condition and produced flowers normally.

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APA: Copy

MAJD, AHMAD, CHAMAN DOUSTI, F., MEHRABIAN, SEDIGHEH, & SHEYDAEI, MASOUD. (2008). SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN CANOLA (BRASSICA NAPUS L.). IRANIAN JOURNAL OF BIOLOGY, 20(4), 344-352. SID. https://sid.ir/paper/21285/en

Vancouver: Copy

MAJD AHMAD, CHAMAN DOUSTI F., MEHRABIAN SEDIGHEH, SHEYDAEI MASOUD. SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN CANOLA (BRASSICA NAPUS L.). IRANIAN JOURNAL OF BIOLOGY[Internet]. 2008;20(4):344-352. Available from: https://sid.ir/paper/21285/en

IEEE: Copy

AHMAD MAJD, F. CHAMAN DOUSTI, SEDIGHEH MEHRABIAN, and MASOUD SHEYDAEI, “SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN CANOLA (BRASSICA NAPUS L.),” IRANIAN JOURNAL OF BIOLOGY, vol. 20, no. 4, pp. 344–352, 2008, [Online]. Available: https://sid.ir/paper/21285/en

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