EXTRACTION AND DETERMINATION OF VANCOMYCIN ANTIBIOTIC IN HOSPITAL SEWAGE SAMPLES BY SOLID PHASE EXTRACTION-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

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SAFAVY S.N.; GHAEMMAGHAMI HEZAVEH S.J.; DEHGHANZADEH RAYHANI R.

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Journal: JOURNAL OF HEALTH AND HYGIENE Year:2014 | Volume:5 | Issue:3 Page(s): 224-234

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Title

EXTRACTION AND DETERMINATION OF VANCOMYCIN ANTIBIOTIC IN HOSPITAL SEWAGE SAMPLES BY SOLID PHASE EXTRACTION-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Author(s)

SAFAVY S.N. | GHAEMMAGHAMI HEZAVEH S.J. | DEHGHANZADEH RAYHANI R. | Issue Writer Certificate

Keywords

VANCOMYCINQ2

HOSPITAL WASTEWATERQ2

LIQUID CHROMATOGRAPHYQ1

SOLID PHASE EXTRACTIONQ3

Abstract

Background & Objectives: VANCOMYCIN, as b-lactam classes of antibiotics, is widely used for treating infectious diseases in hospitals. Therefore, it is necessary to establish a method for precise measurement of VANCOMYCIN in hospital sewage as an emerging pollutant that causes bacterial resistance.Methods: In this study, SOLID PHASE EXTRACTION was used for separation of VANCOMYCIN from HOSPITAL WASTEWATER. VANCOMYCIN recovery rate were investigated at pH 3 and 7. The extracted samples were passed through a C18 column at 20 oC using different ratios of mobile phase of acetate/acetonitrile buffer with flow rate of 0.8 ml min-1. The quantity of VANCOMYCIN in samples was detected by high performance LIQUID CHROMATOGRAPHY (HPLC) equipped with UV detector at 240 nm wave length.Results: The best condition for extraction was achieved at pH=7 with a recovery of 89%. Better results were obtained by mobile phase of acetate/acetonitrile buffer with volume ratio of 89: 11 at pH=4. The linearity coefficients (R2) of 0.97 and 0.96 were obtained for VANCOMYCIN concentration ranges of 1-45 and 50-120 mg l-1 at optimum condition, respectively. Limit of Detection (LOD) and Limit of Quantification (LOQ) were calculated as 3 and 10 mg l-1 respectively. Also, variations in precision of both hourly and daily measurements of standard solution were less than 10%.Conclusion: The results of this study might be applied as a specific method for extraction and analysis of VANCOMYCIN antibiotic in hospital sewage samples.

Cites

INVESTIGATION OF USING FENTON OXIDATION PROCESS FOR AMPICILLIN AND CIPROFLOXACIN REMOVAL FROM HOSPITAL WASTEWATER

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APA: Copy

SAFAVY, S.N., GHAEMMAGHAMI HEZAVEH, S.J., & DEHGHANZADEH RAYHANI, R.. (2014). EXTRACTION AND DETERMINATION OF VANCOMYCIN ANTIBIOTIC IN HOSPITAL SEWAGE SAMPLES BY SOLID PHASE EXTRACTION-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. JOURNAL OF HEALTH AND HYGIENE, 5(3), 224-234. SID. https://sid.ir/paper/226898/en

Vancouver: Copy

SAFAVY S.N., GHAEMMAGHAMI HEZAVEH S.J., DEHGHANZADEH RAYHANI R.. EXTRACTION AND DETERMINATION OF VANCOMYCIN ANTIBIOTIC IN HOSPITAL SEWAGE SAMPLES BY SOLID PHASE EXTRACTION-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. JOURNAL OF HEALTH AND HYGIENE[Internet]. 2014;5(3):224-234. Available from: https://sid.ir/paper/226898/en

IEEE: Copy

S.N. SAFAVY, S.J. GHAEMMAGHAMI HEZAVEH, and R. DEHGHANZADEH RAYHANI, “EXTRACTION AND DETERMINATION OF VANCOMYCIN ANTIBIOTIC IN HOSPITAL SEWAGE SAMPLES BY SOLID PHASE EXTRACTION-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY,” JOURNAL OF HEALTH AND HYGIENE, vol. 5, no. 3, pp. 224–234, 2014, [Online]. Available: https://sid.ir/paper/226898/en

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