EVALUATION OF LIVE ATTENUATEDSHIGELLA DYSENTERIAE TYPE 1 STRAIN AS A CANDIDATE VACCINE

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SALEHI CHALESHTORI A.R.; SAADATI M.; AHSANI ARANI Y.; BAHADOR A.; HOSSEINI M.; HESARAKI M.; DOROUDIAN M.

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Journal: Journal of Police Medicine Year:2012 | Volume:1 | Issue:1 (1) Page(s): 28-35

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Title

EVALUATION OF LIVE ATTENUATEDSHIGELLA DYSENTERIAE TYPE 1 STRAIN AS A CANDIDATE VACCINE

Author(s)

Keywords

SHIGELLA DYSENTERIAE TYPE 1Q4

LIVE ATTENUATEDQ4

KERATOCONJUNCTIVEQ4

GUINEA PIGQ4

VACCINE CANDIDATEQ4

Abstract

Aim: This study aims to detect the ipaD plasmid in SHIGELLA DYSENTERIAE TYPE 1 and evaluate the invasive ability of a live, attenuatedSHIGELLA DYSENTERIAE TYPE 1 generated from deletion of theipaD plasmid. The attenuated strain was tested in GUINEA PIGs.Materials and Methods: We performed biochemical and antibody tests in addition to PCR analysis, to identify the strain and serotype of an isolatedShigella strain. The plasmid version of theipaD gene was recognized. We generated a live, attenuated Shigellaserotype by deletion of the plasmid version of the ipaD gene, which was confirmed by the above mentioned tests. Absence of theipaD gene was assessed in a PCR reaction and compared to the wild type strain. Various dilutions of cultured bacteria were prepared and colony numbers counted. We prepared specific dilution which was appropriate for conducting the KERATOCONJUNCTIVE test. We used 6 GUINEA PIGs that were divided into two groups, control (n=3) and experimental (n=3). C Invasion capability of produced strain was evaluated by KERATOCONJUNCTIVE test in GUINEA PIGs.Results: The produced LIVE ATTENUATED SHIGELLA DYSENTERIAE TYPE 1 was confirmed through serological tests and PCR. Absence ofipaD was confirmed. Results of KERATOCONJUNCTIVE test has shown that this strain lost its ability to invade host cells.Conclusion: The plasmid version of ipaD gene plays a critical role in Shigella invasiveness. Deletion of mentioned gene reduces the invasion ability of these bacteria. Therefore, LIVE ATTENUATED DipaD shigella dysenteriae can be an appropriate candidate to produce vaccine against shigellosis.

Cites

IDENTIFICATION OF IPA AND STX GENES AND THEIR ANTIBIOTIC RESISTANCE PATTERN IN SHIGELLA SONNEI ISOLATED FROM CLINICAL SAMPLES USING MULTIPLEX-PCR

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APA: Copy

SALEHI CHALESHTORI, A.R., SAADATI, M., AHSANI ARANI, Y., BAHADOR, A., HOSSEINI, M., HESARAKI, M., & DOROUDIAN, M.. (2012). EVALUATION OF LIVE ATTENUATEDSHIGELLA DYSENTERIAE TYPE 1 STRAIN AS A CANDIDATE VACCINE. POLICE MEDICINE, 1(1 (1)), 28-35. SID. https://sid.ir/paper/227947/en

Vancouver: Copy

SALEHI CHALESHTORI A.R., SAADATI M., AHSANI ARANI Y., BAHADOR A., HOSSEINI M., HESARAKI M., DOROUDIAN M.. EVALUATION OF LIVE ATTENUATEDSHIGELLA DYSENTERIAE TYPE 1 STRAIN AS A CANDIDATE VACCINE. POLICE MEDICINE[Internet]. 2012;1(1 (1)):28-35. Available from: https://sid.ir/paper/227947/en

IEEE: Copy

A.R. SALEHI CHALESHTORI, M. SAADATI, Y. AHSANI ARANI, A. BAHADOR, M. HOSSEINI, M. HESARAKI, and M. DOROUDIAN, “EVALUATION OF LIVE ATTENUATEDSHIGELLA DYSENTERIAE TYPE 1 STRAIN AS A CANDIDATE VACCINE,” POLICE MEDICINE, vol. 1, no. 1 (1), pp. 28–35, 2012, [Online]. Available: https://sid.ir/paper/227947/en

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