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Information Journal Paper

Title

INDUCTIVE EFFECT OF IDE1 ON DIFFERENTIATION OF HUMAN INDUCED PLURIPOTENT STEM CELLS INTO DEFINITIVE ENDODERM CELLS BY USING PCL NANOFIBROUS SCAFFOLD

Pages

  8-22

Abstract

 Induced pluripotent cells have been considered as one of the most recent and best cell sources for the cell therapy. In this study, the differentiation potency of human iPS cells, cultured on scaffolds, which can differentiate into DEFINITIVE ENDODERMal cells as precursor for hepatocytes, pancreatic and lung cells, was studied. Embryoid bodies composed of pluripotent cells, were seeded on electrospinning nanofiber scaffold.The cells were differentiated into DEFINITIVE ENDODERM using IDE1. Expression of DEFINITIVE ENDODERM markers including Sox17, FoxA2 and GSC were confirmed by immunocytochemistry staining and qRT-PCR analysis. In the present study, morphology and viability of cells were evaluated by utilizing a scanning electron microscopy and MTT assay, respectively. The results demonstrated the positive effect of 3D cultures, using suitable factors, on DEFINITIVE ENDODERM differentiation.

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  • Cite

    APA: Copy

    HOVEIZI, ELHAM, NABIUNI, MOHAMMAD, PARIVAR, KAZEM, MASSUMI, MOHAMMAD, & AI, JAFAR. (2014). INDUCTIVE EFFECT OF IDE1 ON DIFFERENTIATION OF HUMAN INDUCED PLURIPOTENT STEM CELLS INTO DEFINITIVE ENDODERM CELLS BY USING PCL NANOFIBROUS SCAFFOLD. NOVA BIOLOGICA REPERTA, 1(1), 8-22. SID. https://sid.ir/paper/250501/en

    Vancouver: Copy

    HOVEIZI ELHAM, NABIUNI MOHAMMAD, PARIVAR KAZEM, MASSUMI MOHAMMAD, AI JAFAR. INDUCTIVE EFFECT OF IDE1 ON DIFFERENTIATION OF HUMAN INDUCED PLURIPOTENT STEM CELLS INTO DEFINITIVE ENDODERM CELLS BY USING PCL NANOFIBROUS SCAFFOLD. NOVA BIOLOGICA REPERTA[Internet]. 2014;1(1):8-22. Available from: https://sid.ir/paper/250501/en

    IEEE: Copy

    ELHAM HOVEIZI, MOHAMMAD NABIUNI, KAZEM PARIVAR, MOHAMMAD MASSUMI, and JAFAR AI, “INDUCTIVE EFFECT OF IDE1 ON DIFFERENTIATION OF HUMAN INDUCED PLURIPOTENT STEM CELLS INTO DEFINITIVE ENDODERM CELLS BY USING PCL NANOFIBROUS SCAFFOLD,” NOVA BIOLOGICA REPERTA, vol. 1, no. 1, pp. 8–22, 2014, [Online]. Available: https://sid.ir/paper/250501/en

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