STUDY OF FIBROBLAST CELLS BEHAVIOR ON SUPRAMOLECULAR POLYCAPROLACTONE NANOFIBERS COATED WITH GELATIN

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SHAKERI SHEMIRANI ZAHRA; ZANDI MOJGAN; SHOKROLLAHI PARVIN; IRANI SHIVA

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Title

STUDY OF FIBROBLAST CELLS BEHAVIOR ON SUPRAMOLECULAR POLYCAPROLACTONE NANOFIBERS COATED WITH GELATIN

Author(s)

SHAKERI SHEMIRANI ZAHRA | ZANDI MOJGAN | SHOKROLLAHI PARVIN | IRANI SHIVA | Issue Writer Certificate

Keywords

TISSUE ENGINEERINGQ2

NANOFIBERSQ2

POLY CAPROLACTONEQ2

SUPRAMOLECULARQ2

GELATINQ2

FIBROBLAST L929 CELL LINEQ2

Abstract

Background: TISSUE ENGINEERING has the potential to create organ and tissue in the form of de novo. Scaffolds are based on structures existing in the extracellular matrix. The trend of successful use of scaffolds is growing rapidly in organ transplantation and in order to expedite such successful use, structures similar to the target tissue are to be used.The coverage of PCL with GELATIN is devised to enable the surface properties of polymer to have hydrophilicity and permeability.This study is to investigate the cell behavior towards GELATIN-coated poly-caprolactone SUPRAMOLECULAR NANOFIBERS polymer.Methods: Poly-caprolactone SUPRAMOLECULAR NANOFIBERS were prepared by electrospinning method, and were coated with GELATIN. Poly-caprolactone SUPRAMOLECULAR covered with GELATIN has been investigated by FTIR, and scaffolds morphology has been investigated by scanning electron microscope (SEM). L929 fibroblast cells have been cultured on scaffolds, and their cellular activity has been assessed first by MTT test and then has been stained with DAPI to confirm the presence of cells on the NANOFIBERS.Results: The results showed that L929 fibroblast cells, when cultured on NANOFIBERS, such NANOFIBERS can create three-dimensional topography to support the cell growth and cell proliferation, GELATIN-coated poly-caprolactone SUPRAMOLECULAR NANOFIBERS has had impact on their biocompatibility. Meantime, MTT assay has confirmed the biocompatibility of the resulting graph.Conclusion: GELATIN- coated poly-caprolactone SUPRAMOLECULAR NANOFIBERS, leads to improved surface properties of polymer and to suitable cell behavior.

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APA: Copy

SHAKERI SHEMIRANI, ZAHRA, ZANDI, MOJGAN, SHOKROLLAHI, PARVIN, & IRANI, SHIVA. (2015). STUDY OF FIBROBLAST CELLS BEHAVIOR ON SUPRAMOLECULAR POLYCAPROLACTONE NANOFIBERS COATED WITH GELATIN. JOURNAL OF CLINICAL RESEARCH IN PARAMEDICAL SCIENCES, 4(1), 1-13. SID. https://sid.ir/paper/256040/en

Vancouver: Copy

SHAKERI SHEMIRANI ZAHRA, ZANDI MOJGAN, SHOKROLLAHI PARVIN, IRANI SHIVA. STUDY OF FIBROBLAST CELLS BEHAVIOR ON SUPRAMOLECULAR POLYCAPROLACTONE NANOFIBERS COATED WITH GELATIN. JOURNAL OF CLINICAL RESEARCH IN PARAMEDICAL SCIENCES[Internet]. 2015;4(1):1-13. Available from: https://sid.ir/paper/256040/en

IEEE: Copy

ZAHRA SHAKERI SHEMIRANI, MOJGAN ZANDI, PARVIN SHOKROLLAHI, and SHIVA IRANI, “STUDY OF FIBROBLAST CELLS BEHAVIOR ON SUPRAMOLECULAR POLYCAPROLACTONE NANOFIBERS COATED WITH GELATIN,” JOURNAL OF CLINICAL RESEARCH IN PARAMEDICAL SCIENCES, vol. 4, no. 1, pp. 1–13, 2015, [Online]. Available: https://sid.ir/paper/256040/en

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