EVALUATION OF HISTOCHEMICAL CHANGES OF MICE OVARIAN TISSUE AFTER USE OF RUTA GRAVEOLENS AQUEOUS EXTRACT

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Hoshyari Aref; ZAREI LEILA; NAJAFI GHOLAMREZA

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Journal: JOURNAL OF NEYSHABUR UNIVERSITY OF MEDICAL SCIENCES Year:2016 | Volume:4 | Issue:3 Page(s): 38-47

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Title

EVALUATION OF HISTOCHEMICAL CHANGES OF MICE OVARIAN TISSUE AFTER USE OF RUTA GRAVEOLENS AQUEOUS EXTRACT

Author(s)

Hoshyari Aref | ZAREI LEILA | NAJAFI GHOLAMREZA | Issue Writer Certificate

Keywords

MICE OVARYQ2

RUTAGRAVEOLENSQ1

ALPQ2

PASQ2

SUDAN BLACKQ1

Abstract

Introduction and Aims: Ruta Graveolens (RG) has been used in various medical preparations for its anti-oxidant, anti-inflammatory, anti-tumour, anti-androgenic and anti-fertility properties. More than 120 natural compounds mainly including acridone alkaloids, coumarines, essential oils, flavonoids, and furoquinolines have been found in the roots and aerial parts of this plant. The aim of this work is to study the anti-fertility propertiese of RG extract. Histochemical studies have been carried out in mouse ovary.Materials and Methods: In this study 36 female mice were used in two groups as control and RG. Control group received the saline normal 0.2 ml and the RG group received 300mg/kg of the aqueous extract of RG per day orally for 14 days. Ovaries were studied after staining ALP, PAS and SUDAN BLACK.Results: It was found that in RG group of first week most follicle were atretic. Results indicate that the staining intensity in ALP, PAS and SUDAN BLACK were sever in RG group when compared with the control group. There was no significant difference between the control and RG group after 2 and 3 weeks treatment.Conclusion: RG extract caused metabolic disorder and reduced fertility. This may be due to the increase in the intensity of reaction due to the accumulation of lidids and carbohydrates and damage of intracytoplasmic organels.

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APA: Copy

Hoshyari, Aref, ZAREI, LEILA, & NAJAFI, GHOLAMREZA. (2016). EVALUATION OF HISTOCHEMICAL CHANGES OF MICE OVARIAN TISSUE AFTER USE OF RUTA GRAVEOLENS AQUEOUS EXTRACT. JOURNAL OF NEYSHABUR UNIVERSITY OF MEDICAL SCIENCES, 4(3 ), 38-47. SID. https://sid.ir/paper/264431/en

Vancouver: Copy

Hoshyari Aref, ZAREI LEILA, NAJAFI GHOLAMREZA. EVALUATION OF HISTOCHEMICAL CHANGES OF MICE OVARIAN TISSUE AFTER USE OF RUTA GRAVEOLENS AQUEOUS EXTRACT. JOURNAL OF NEYSHABUR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;4(3 ):38-47. Available from: https://sid.ir/paper/264431/en

IEEE: Copy

Aref Hoshyari, LEILA ZAREI, and GHOLAMREZA NAJAFI, “EVALUATION OF HISTOCHEMICAL CHANGES OF MICE OVARIAN TISSUE AFTER USE OF RUTA GRAVEOLENS AQUEOUS EXTRACT,” JOURNAL OF NEYSHABUR UNIVERSITY OF MEDICAL SCIENCES, vol. 4, no. 3 , pp. 38–47, 2016, [Online]. Available: https://sid.ir/paper/264431/en

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