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Information Journal Paper

Title

SEROTYPES OF ENTEROPATHOGENIC ESCHERICHIA COLI ISOLATED FROM CHILDREN UNDER 5 YEARS OF AGE

Pages

  70-77

Abstract

 Background: The purpose of this study was to find out the frequency of different serotypes of enteropathogenic Escherichia coli (EPEC) among healthy/diarrheal cases.Methods: A total of 191 strains, 111 from DIARRHEAl and 80 from asymptomatic persons were examined. Determination of the EPEC serogroups was performed by agglutination tests using polyvalent and monovalent O antiserum. PCR-RFLP analysis of the flagellin-encoding (fliC) gene and agglutination tests using polyvalent and monovalent sera against H antigens (H1 to H 56) according to the instructions of the manufacturer was performed.Results: Seventeen (8.9%) strains were non-motile and untypable with conventional serotyping method that showed as H-. Forty-three fliC restriction patterns were found for motile and non-motile serotypes. Each motile serotype was characterized by one or two fliC specific restriction patterns. O142:H48 (6.8%), O86:H48 (6.3%), O111:H21 (4.7%) and O127:H21 (4.2%) were the most prevalent serotypes, and O55:H12/45, O86:H48, O127:H21, O142:H48, O126:H48 and O126:H19 serotypes were the most frequently agents in DIARRHEAl cases, compared to asymptomatic children (P< 0.05). There were common EPEC SEROTYPES in DIARRHEAl and asymptomatic children, however some serotypes either found only in DIARRHEAl cases or isolated from asymptomatic persons.Conclusion: Some serotypes were isolated more frequently from DIARRHEAl cases than asymptomatic persons. The conventional serological method using antisera is the basis for the H typing system in E-coli, but it is impossible to serotype nonmotile bacteria. PCR-RFLP analysis of FLIC GENE is a practical method in identifying the H variant in motile and non-motile EPEC SEROTYPES and is useful for epidemiological studies.

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    APA: Copy

    ASLANI, M.M., & ALIKHANI, M.Y.. (2009). SEROTYPES OF ENTEROPATHOGENIC ESCHERICHIA COLI ISOLATED FROM CHILDREN UNDER 5 YEARS OF AGE. IRANIAN JOURNAL OF PUBLIC HEALTH, 38(3), 70-77. SID. https://sid.ir/paper/272675/en

    Vancouver: Copy

    ASLANI M.M., ALIKHANI M.Y.. SEROTYPES OF ENTEROPATHOGENIC ESCHERICHIA COLI ISOLATED FROM CHILDREN UNDER 5 YEARS OF AGE. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2009;38(3):70-77. Available from: https://sid.ir/paper/272675/en

    IEEE: Copy

    M.M. ASLANI, and M.Y. ALIKHANI, “SEROTYPES OF ENTEROPATHOGENIC ESCHERICHIA COLI ISOLATED FROM CHILDREN UNDER 5 YEARS OF AGE,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 38, no. 3, pp. 70–77, 2009, [Online]. Available: https://sid.ir/paper/272675/en

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