PRODUCTION AND EVALUATION OF [67GA]-DTPA-RITUXIMAB

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JALILIAN A.R.; MIRSADEGH L.; HAJHOSSEINI R.; RAJABIFAR S.; BOLURINOVIN F.

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Journal Paper

Paper Information

Journal: INTERNATIONAL JOURNAL OF RADIATION RESEARCH Year:2007 | Volume:4 | Issue:4 Page(s): 187-193

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Title

PRODUCTION AND EVALUATION OF [67GA]-DTPA-RITUXIMAB

Author(s)

Keywords

GALLIUM-67

RITUXIMAB

RADIOLABELING

BIODISTRIBUTION

CYCLOTRON

Abstract

Background: In order to obtain an anti-CD2O conjugate to be used in future therapeutic studies with therapeutic radioisotopes, 67Ga-labeled antibody wasprepared as a model of metal chelated immunoconjugate for preliminary dosimetric and biodistribution studies. Materials and Methods: Rituximab was labeled with [67Ga]-gallium chloride after residulation with freshly prepared cyclic DTPAdianhydride. The best results of the conjugation were obtained by the addition of 1 ml of a rituximab pharmaceutical solution (5 mg/ml, in phosphate buffer, pH=8) to a glass tube pre-coated with DTPA- dianhydride (0.01 mg) at 25oC with continuous mild stirring for 30 min. The final isotonic 67Ga-DTPArituximab complex was checked by gel electrophoresis for radiolysis/chemolysis control. Radio-TLC was performed to ensure the formation of only one species. Preliminary in vivo studies in normal rat model were performed to determine the biodistribution of the radioimmunoconjugate up to 6 hours. Results: Radio-thin layer chromatography showed an overall radiochemical purity of 96-99% at optimized conditions (specific activity =300-500 MBq/mg, labeling efficiency 77%). Gel electrophoresis showed no protein cleavage after radiolabeling. Conclusion: Preliminary in vivo studies in normal rat model showed [67Ga]-DTPA-rituximab is a good probe for bio-dosimetry of therapeutic rituximab conjugates.

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APA: Copy

JALILIAN, A.R., MIRSADEGH, L., HAJHOSSEINI, R., RAJABIFAR, S., & BOLURINOVIN, F.. (2007). PRODUCTION AND EVALUATION OF [67GA]-DTPA-RITUXIMAB . INTERNATIONAL JOURNAL OF RADIATION RESEARCH, 4(4), 187-193. SID. https://sid.ir/paper/286403/en

Vancouver: Copy

JALILIAN A.R., MIRSADEGH L., HAJHOSSEINI R., RAJABIFAR S., BOLURINOVIN F.. PRODUCTION AND EVALUATION OF [67GA]-DTPA-RITUXIMAB . INTERNATIONAL JOURNAL OF RADIATION RESEARCH[Internet]. 2007;4(4):187-193. Available from: https://sid.ir/paper/286403/en

IEEE: Copy

A.R. JALILIAN, L. MIRSADEGH, R. HAJHOSSEINI, S. RAJABIFAR, and F. BOLURINOVIN, “PRODUCTION AND EVALUATION OF [67GA]-DTPA-RITUXIMAB ,” INTERNATIONAL JOURNAL OF RADIATION RESEARCH, vol. 4, no. 4, pp. 187–193, 2007, [Online]. Available: https://sid.ir/paper/286403/en

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