IN-VITRO CELLULAR UPTAKE AND TRANSPORT STUDY OF 9-NITROCAMPTOTHECIN PLGA NANOPARTICLES ACROSS CACO-2 CELL MONOLAYER MODEL

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DERAKHSHANDEH KATAYOUN; HOCHHAUS GUNTHER; DADASHZADEH SIMIN

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Journal Paper

Paper Information

Journal: Iranian Journal of Pharmaceutical Research Year:2011 | Volume:10 | Issue:3 Page(s): 425-434

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Information Journal Paper

Title

IN-VITRO CELLULAR UPTAKE AND TRANSPORT STUDY OF 9-NITROCAMPTOTHECIN PLGA NANOPARTICLES ACROSS CACO-2 CELL MONOLAYER MODEL

Author(s)

DERAKHSHANDEH KATAYOUN | HOCHHAUS GUNTHER | DADASHZADEH SIMIN | Issue Writer Certificate

Keywords

9-NITROCAMPTOTHECIN

NANOPARTICLES

UPTAKE

TRANSPORT

CACO-2 CELL

Abstract

The UPTAKE and TRANSPORT of 9-NITROCAMPTOTHECIN (9-NC), a potent anticancer agent, across CACO-2 CELL monolayers was studied as a free and PLGA nanoparticle loaded drug.Different sizes (110 to 950 nm) of 9-NITROCAMPTOTHECIN NANOPARTICLES using poly (lacticglycolic acid) were prepared by via the nanoprecipitation method. The TRANSPORT of NANOPARTICLES across the CACO-2 CELL monolayer as a function of incubation time and concentration was evaluated for each different nanoparticle formulation. The amount of 9-NC TRANSPORTed from the apical to the basolateral side and the UPTAKE of the drug into the cells was determined by HPLC.The UPTAKE of intact NANOPARTICLES into CACO-2 CELLs was visualized by confocal laser scanning microscopy using 6-coumarin as a fluorescent marker. The study demonstrated that CACO-2 CELL UPTAKE and TRANSPORT of encapsulated 9-NITROCAMPTOTHECIN is significantly affected by the diameter of the carrier and incubation time. In addition it was shown to be independent of concentration.The results indicated a significant accumulation of the drug in the cell membrane and an enhanced diffusion across the cell membrane. There was also a sustained release of characteristics pertaining to polymeric carriers that provided prolonged drug availability for absorptive cells.

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APA: Copy

DERAKHSHANDEH, KATAYOUN, HOCHHAUS, GUNTHER, & DADASHZADEH, SIMIN. (2011). IN-VITRO CELLULAR UPTAKE AND TRANSPORT STUDY OF 9-NITROCAMPTOTHECIN PLGA NANOPARTICLES ACROSS CACO-2 CELL MONOLAYER MODEL. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 10(3), 425-434. SID. https://sid.ir/paper/287842/en

Vancouver: Copy

DERAKHSHANDEH KATAYOUN, HOCHHAUS GUNTHER, DADASHZADEH SIMIN. IN-VITRO CELLULAR UPTAKE AND TRANSPORT STUDY OF 9-NITROCAMPTOTHECIN PLGA NANOPARTICLES ACROSS CACO-2 CELL MONOLAYER MODEL. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2011;10(3):425-434. Available from: https://sid.ir/paper/287842/en

IEEE: Copy

KATAYOUN DERAKHSHANDEH, GUNTHER HOCHHAUS, and SIMIN DADASHZADEH, “IN-VITRO CELLULAR UPTAKE AND TRANSPORT STUDY OF 9-NITROCAMPTOTHECIN PLGA NANOPARTICLES ACROSS CACO-2 CELL MONOLAYER MODEL,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 10, no. 3, pp. 425–434, 2011, [Online]. Available: https://sid.ir/paper/287842/en

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