IN VITRO GENERATION OF IL-35-EXPRESSING HUMAN WHARTON’S JELLY-DERIVED MESENCHYMAL STEM CELLS USING LENTIVIRAL VECTOR

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AMARI AFSHIN; EBTEKAR MASSOUMEH; MOAZZENI SEYED MOHAMMAD; SOLEIMANI MASOUD; MOHAMMADI AMIRABAD LEILA; TAHOORI MOHAMMAD TAHER; MASSUMI MOHAMMAD

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Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2015 | Volume:14 | Issue:4 Page(s): 416-426

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Title

IN VITRO GENERATION OF IL-35-EXPRESSING HUMAN WHARTON’S JELLY-DERIVED MESENCHYMAL STEM CELLS USING LENTIVIRAL VECTOR

Author(s)

Keywords

IL-35

LENTIVIRUS

MESENCHYMAL STEM CELLS

VECTOR

WHARTON’S JELLY-DERIVED

Abstract

Human WHARTON’S JELLY-DERIVED MESENCHYMAL STEM CELLS (hWJ-MSCs) are easily available cells without transplant rejection problems or ethical concerns compared to bone-marrow-derived MSCs for prospective clinical applications. These cells display immunosuppressive properties and may be able to play an important role in autoimmune disorders. Regulatory T-cells (Treg) are important to prevent autoimmune disease development. Interleukin 35 (IL-35) induces the proliferation of Treg cell populations and reduces the activity of T helper 17 (Th17) and T helper 1 (Th1) cells, which play a central role in initiation of inflammation and autoimmune disease.Recent studies identified IL-35 as a new inhibitory cytokine required for the suppressive function of Treg cells. We created IL-35-producing hWJ-MSCs as a good vehicle for reduction of inflammation and autoimmune diseases. We isolated hWJ-MSCs based on explant culture. HWJ-MSCs were transduced at MOI=50 (Multiplicity of Infection) with lentiviral particles harboring murine Interleukin 35 (mIL-35). Expression of IL-35 in hWJ-MSCs was quantified by an IL-35 ELISA kit. IL-35 bioactivity was analyzed by inhibiting the proliferation of mouse splenocytes using CFSE cell proliferation kit. Frequency of CD4+CD25+CD127low/neg Foxp3+ Treg cells was measured by flow cytometry. There was an up to 85% GFP positive transduction rate, and the cells successfully released a high level of mIL-35 protein (750 ng/ml). IL-35 managed to inhibit CD4+ T cell proliferation with PHA, and improved the frequency of Treg cells. Our data suggest that transduced hWJ-MSCs overexpressing IL-35 may provide a useful approach for basic research on gene therapy for autoimmune disorders.

Cites

THE EFFECT OF TRANSPLANTED HUMAN WHARTON’S JELLY MESENCHYMAL STEM CELLS ON MATRIX METALLOPROTEINASES IN BRAIN OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS MICE

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APA: Copy

AMARI, AFSHIN, EBTEKAR, MASSOUMEH, MOAZZENI, SEYED MOHAMMAD, SOLEIMANI, MASOUD, MOHAMMADI AMIRABAD, LEILA, TAHOORI, MOHAMMAD TAHER, & MASSUMI, MOHAMMAD. (2015). IN VITRO GENERATION OF IL-35-EXPRESSING HUMAN WHARTON’S JELLY-DERIVED MESENCHYMAL STEM CELLS USING LENTIVIRAL VECTOR. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 14(4), 416-426. SID. https://sid.ir/paper/291476/en

Vancouver: Copy

AMARI AFSHIN, EBTEKAR MASSOUMEH, MOAZZENI SEYED MOHAMMAD, SOLEIMANI MASOUD, MOHAMMADI AMIRABAD LEILA, TAHOORI MOHAMMAD TAHER, MASSUMI MOHAMMAD. IN VITRO GENERATION OF IL-35-EXPRESSING HUMAN WHARTON’S JELLY-DERIVED MESENCHYMAL STEM CELLS USING LENTIVIRAL VECTOR. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2015;14(4):416-426. Available from: https://sid.ir/paper/291476/en

IEEE: Copy

AFSHIN AMARI, MASSOUMEH EBTEKAR, SEYED MOHAMMAD MOAZZENI, MASOUD SOLEIMANI, LEILA MOHAMMADI AMIRABAD, MOHAMMAD TAHER TAHOORI, and MOHAMMAD MASSUMI, “IN VITRO GENERATION OF IL-35-EXPRESSING HUMAN WHARTON’S JELLY-DERIVED MESENCHYMAL STEM CELLS USING LENTIVIRAL VECTOR,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 14, no. 4, pp. 416–426, 2015, [Online]. Available: https://sid.ir/paper/291476/en

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