PREPARATION AND STANDARDIZATION OF LEISHMANIA SUSPENSION AND ITS EVALUATION FOR LEISHMANIAZATION IN A LABORATORY ANIMAL MODEL

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MOHEBALI M.; MEHRABI TAVANA A.; JAVADIAN E.; ESFAHANI A.A.; HAJARAN H.; AKHOUNDI B.

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Journal: HAKIM RESEARCH JOURNAL Year:2003 | Volume:6 | Issue:3 Page(s): 15-19

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Title

PREPARATION AND STANDARDIZATION OF LEISHMANIA SUSPENSION AND ITS EVALUATION FOR LEISHMANIAZATION IN A LABORATORY ANIMAL MODEL

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STANDARD LEISHMANIA SUSPENSIONQ4

EVALUATIONQ2

ANIMAL MODELQ4

Abstract

Introduction: This study was designed for preparation and EVALUATION of STANDARD LEISHMANIA SUSPENSION against small-white mice (out-bred(.Methods: Leishmania suspension was prepared from Leishmania major (MRHO/IR/75/ER). The Leishmania parasites were cultured in Schneider and RPMI1640 culture media. We then, subcultured them for each 72 hours interval. The promastigotes were harvested 120 hours post the fourth subculture and washed with isotonic normal saline. The parasite suspension was mixed with phosphate buffer saline (PBS, pH=7.2) or isotonic glucose- Nacl and glycerole 10% and counted by Neobar procedure (2X107 parasite /ml) and then were gradually freezed in liquid nitrogen. Viability test showed that from 5 to 25% of promastigotes were alive after this process. Two hundred and thirty seven mice (outbred-souri) were divided to two groups. Group 1 (135) were inoculated by 0.1 ml of Leishmania suspension subcutaneously in their base of tail. The mice of control group (102) were injected by isotonic normal saline with the same procedure. Results: Lesions were manifested from one to 6 months post inoculation. Fifty eight (63%) of the rest mice from interventional group, were infected versus none of the mice from control group showed any lesion. Leishmanin skin test (LST) conversion rate was significant higher in interventional group compare, to control group after 72 hours. (54.1% VS 4.3%, P>0.05).Conclusion: In conclusion, this study showed the standard Leismania suspension can produce lesion and provoke the CMI in the small-white mice.

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APA: Copy

MOHEBALI, M., MEHRABI TAVANA, A., JAVADIAN, E., ESFAHANI, A.A., HAJARAN, H., & AKHOUNDI, B.. (2003). PREPARATION AND STANDARDIZATION OF LEISHMANIA SUSPENSION AND ITS EVALUATION FOR LEISHMANIAZATION IN A LABORATORY ANIMAL MODEL. HAKIM RESEARCH JOURNAL, 6(3), 15-19. SID. https://sid.ir/paper/29325/en

Vancouver: Copy

MOHEBALI M., MEHRABI TAVANA A., JAVADIAN E., ESFAHANI A.A., HAJARAN H., AKHOUNDI B.. PREPARATION AND STANDARDIZATION OF LEISHMANIA SUSPENSION AND ITS EVALUATION FOR LEISHMANIAZATION IN A LABORATORY ANIMAL MODEL. HAKIM RESEARCH JOURNAL[Internet]. 2003;6(3):15-19. Available from: https://sid.ir/paper/29325/en

IEEE: Copy

M. MOHEBALI, A. MEHRABI TAVANA, E. JAVADIAN, A.A. ESFAHANI, H. HAJARAN, and B. AKHOUNDI, “PREPARATION AND STANDARDIZATION OF LEISHMANIA SUSPENSION AND ITS EVALUATION FOR LEISHMANIAZATION IN A LABORATORY ANIMAL MODEL,” HAKIM RESEARCH JOURNAL, vol. 6, no. 3, pp. 15–19, 2003, [Online]. Available: https://sid.ir/paper/29325/en

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