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Information Journal Paper

Title

The Effect of Rutin on Progesterone and Estrogen Receptor Expression in Uterine Endometrial Tissue in the Heterotopic Transplantation of Newborn Mouse Ovary

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Abstract

 Background: Rutin (quercetin-3-rhamnosyl-glucoside), a flavonoid, is derived from plants and has antioxidant properties. Objectives: This study aimed to evaluate the effect of different concentrations of Rutin on mouse Ovary heterotopic alloTransplantation. Methods: The present animal experimental study was conducted on 40 female adult Balb/c Mice weighing 30 5 g at the Jundishapur University of Medical Sciences, Ahvaz, Iran, during 2016-2018. The Mice were divided by permuted block randomization into 8 groups (n = 5): OVX (ovariectomy), as the negative control; normal (positive control); OVX + OVA (ovariectomy and Transplantation) (control), treated with 0. 5mLof normal saline; OVX + OVA + 10 mg/kg of Rutin; OVX + OVA + 30 mg/kg of Rutin; OVX + OVA + 60 mg/kg of Rutin; OVX + OVA + 100 mg/kg of Rutin; and the autograft. Groups were treated daily. Fourteen days after Transplantation, ovarian grafts were collected and processed histologically for Follicle number counting. Serum estrogen (E2) and Progesterone (P4) levels were evaluated. Furthermore, the expression of Estrogen Receptor Alpha (ER ), Estrogen Receptor Beta (ER ), and Progesterone Receptor (PR) in the uterine endometrial tissue was tested using qRT-PCR and Western Blotting. Results: A decrease in the number of mature Follicles and increase in the number of atretic Follicles (mean SD: OVX + OVA + 30 = 19. 00 1. 000, OVX + OVA + 60 = 25. 00 5. 000, and OVX + OVA + 100 = 23. 00 2. 646) were observed in all groups treated with Rutin in comparison with the control group (mean  SD: 12. 33  2. 517) (P value < 0. 05). The level of E2 and P4 (mean  SD: OVX + OVA + 100 = 6. 133 1. 026) increased in comparison with the OVX + OVA group (mean SD: 0. 4667 0. 2517) (P value < 0. 05). The protein expression of ER (mean SD: OVX + OVA + 10 = 1. 615 0. 1701 and OVX + OVA + 30 = 1. 744 0. 1779) in comparison with the control group (mean SD: 0. 7089 0. 1131), and ER (mean SD: OVX + OVA + 10 = 0. 7747 0. 4365, OVX + OVA + 30 = 0. 9220 0. 1245, OVX + OVA + 60 = 0. 7701 0. 2150, and OVX + OVA + 100 = 0. 6676 0. 1547) increased in a dose-dependent manner in all groups treated with Rutin in comparison with the OVX + OVA group (mean SD: 0. 1534 0. 06109) (P value < 0. 05). No significant changes in PR were found in groups treated with Rutin in comparison with the control group. Conclusions: The results of the present study indicated that Rutin increases E2 and P4 levels in ovarian hetero allograft Mice. Rutin also upregulated the expression of ER and ER but had no significant effect on PR.

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    APA: Copy

    Hadigol, Tayebeh, SOBHANI, ALIGHOLI, HEMADI, MASOUD, NEKOONAM, SAEID, SHAMS, ALIREZA, ESLAMI FARSANI, BAHRAM, DASTOORPOOR, MARYAM, & SAKI, GHASEM. (2019). The Effect of Rutin on Progesterone and Estrogen Receptor Expression in Uterine Endometrial Tissue in the Heterotopic Transplantation of Newborn Mouse Ovary. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), 21(4), 0-0. SID. https://sid.ir/paper/293883/en

    Vancouver: Copy

    Hadigol Tayebeh, SOBHANI ALIGHOLI, HEMADI MASOUD, NEKOONAM SAEID, SHAMS ALIREZA, ESLAMI FARSANI BAHRAM, DASTOORPOOR MARYAM, SAKI GHASEM. The Effect of Rutin on Progesterone and Estrogen Receptor Expression in Uterine Endometrial Tissue in the Heterotopic Transplantation of Newborn Mouse Ovary. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ)[Internet]. 2019;21(4):0-0. Available from: https://sid.ir/paper/293883/en

    IEEE: Copy

    Tayebeh Hadigol, ALIGHOLI SOBHANI, MASOUD HEMADI, SAEID NEKOONAM, ALIREZA SHAMS, BAHRAM ESLAMI FARSANI, MARYAM DASTOORPOOR, and GHASEM SAKI, “The Effect of Rutin on Progesterone and Estrogen Receptor Expression in Uterine Endometrial Tissue in the Heterotopic Transplantation of Newborn Mouse Ovary,” IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), vol. 21, no. 4, pp. 0–0, 2019, [Online]. Available: https://sid.ir/paper/293883/en

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