COMPARISON OF SURVIVAL RATE AND DEVELOPMENTAL COMPETENCE OF MOUSE MII OOCYTE AFTER FREEZING BY SLOW FREEZING AND VITRIFICATION

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AGHA RAHIMI A.; KALANTAR S.M.; AZARNIA MAHNAZ; SOLEYMANI M.; MIRESMAEILI SEYED MOHSEN

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Journal: INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE Year:2009 | Volume:7 | Issue:SUPPL 2 Page(s): 0-0

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Title

COMPARISON OF SURVIVAL RATE AND DEVELOPMENTAL COMPETENCE OF MOUSE MII OOCYTE AFTER FREEZING BY SLOW FREEZING AND VITRIFICATION

Author(s)

AGHA RAHIMI A. | KALANTAR S.M. | AZARNIA MAHNAZ | SOLEYMANI M. | MIRESMAEILI SEYED MOHSEN

Keywords

VITRIFICATION

SLOW FREEZING

SURVIVAL RATE

MII OOCYTE

IN VITRO FERTILIZATION

Abstract

Introduction: Oocyte freezing is the only method to preserve the reproductive capacity for woman at risk of losing it because of premature ovarian failure, pelvic diseases, surgery, radiotherapy or chemotherapy treatments related to the ovary . But oocyte storage has faced technical difficulties compared with sperm or embryo cryopreservation because of specific structure of oocyte. The purpose of this study is investigated the morphological survival rate and developmental competence of thawed oocytes after cryopreservation by slow freezing or vitrification.Materials and Methods: Female mice superovulated with intraperitoneal injection of PMSG and HCG. Ovulated MII mouse oocytes were allocated to the three groups as slow frozen, vitrified and control groups. Vitrification performed using ethylene glycol (EG) and dimethyl solfoxide (DMSO) and slow freezing using propanediol (PROH). After thawing the surviving MII oocytes in both cryopreserved and control group were inseminated for in vitro fertilization (IVF) and their developmental ability were compared.Results: The survival rate of post thawed mature oocytes in vitrification group was 73.3% and in the slow freezing was 33.3%. Then following IVF, the percentage of cleaved embryos in control, vitrification and slow freezing groups was 64%, 25% and 20%, respectively.Conclusion: The result showed that survival rate after thawing in vitrification was greater than slow freezing while the cleave embryo rate did not. Vitrification seems to be an effective, easy and rapid method for the cryopreservation of mouse MII oocytes.

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APA: Copy

AGHA RAHIMI, A., KALANTAR, S.M., AZARNIA, MAHNAZ, SOLEYMANI, M., & MIRESMAEILI, SEYED MOHSEN. (2009). COMPARISON OF SURVIVAL RATE AND DEVELOPMENTAL COMPETENCE OF MOUSE MII OOCYTE AFTER FREEZING BY SLOW FREEZING AND VITRIFICATION. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 7(SUPPL 2), 0-0. SID. https://sid.ir/paper/295441/en

Vancouver: Copy

AGHA RAHIMI A., KALANTAR S.M., AZARNIA MAHNAZ, SOLEYMANI M., MIRESMAEILI SEYED MOHSEN. COMPARISON OF SURVIVAL RATE AND DEVELOPMENTAL COMPETENCE OF MOUSE MII OOCYTE AFTER FREEZING BY SLOW FREEZING AND VITRIFICATION. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2009;7(SUPPL 2):0-0. Available from: https://sid.ir/paper/295441/en

IEEE: Copy

A. AGHA RAHIMI, S.M. KALANTAR, MAHNAZ AZARNIA, M. SOLEYMANI, and SEYED MOHSEN MIRESMAEILI, “COMPARISON OF SURVIVAL RATE AND DEVELOPMENTAL COMPETENCE OF MOUSE MII OOCYTE AFTER FREEZING BY SLOW FREEZING AND VITRIFICATION,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 7, no. SUPPL 2, pp. 0–0, 2009, [Online]. Available: https://sid.ir/paper/295441/en

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