مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

338
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

132
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

2

Information Journal Paper

Title

MULTIPLEX PCR SCREENING OF Y-CHROMOSOME MICRODELETIONS IN AZOOSPERMIC ICSI CANDIDATE MEN

Pages

  335-338

Abstract

 Abstract Background: It has been hypothesized that Y-q microdeletion can account for significant proportion of infertility in men. There are three nonoverlapping regions referred to as the “azoozpermia factors” AZFa, AZFb, and AZFc from proximal to distal part of Y-q. These have been defined as spermatogenesis loci, this region deletions have been shown to be involved in male azoospermic or severe oligoozospermic infertility.Objective: Evaluation the rate of Y-chromosome microdeletions in infertile men. Materials and Methods: In this case-control study, 25 azoospermic infertile men candidate for intracytoplasmic sperm injection (ICSI) were selected as case group. For control group, 25 normoozoospemric men were selected. All cases and controls had normal 46XY karyotype. DNA extraction and molecular analysis were done on blood samples. Multiplex-PCR method was done to identify the presence of microdeletion in AZFa, AZFb or AZFc loci. Eight STS primers that include two controls were selected to determine Y-chromosome microdeletions.Results: 20% (5.25) of all patients have at least one microdeletion in more than one region of AZF loci. Totally 17 microdeletions was observed, one case had deletions in three AZF regions, and 4 cases had deletions in two AZF regions. The rate of deletions was 42% (7.17) for AZFc, 35% (6.17) for AZFa and 23% (4.17) for AZFb.Conclusion: The molecular DNA analysis could help us to know the real cause of infertility and can give good information for good decision for example in men whit microdeletions who want to undertake ICSI procedure the deletions will be passed to their son.

Cites

References

  • No record.

    • Cite

    APA: Copy

    SHEIKHHA, MOHAMMAD HASAN, ZAIMY, MOHAMMAD ALI, SOLEIMANIAN, SAEEDE, KALANTAR, SEYED MEHDI, RASTI, AZAM, GOLZADE, MARYAM, & HOSEINI FAHRAJI, HAMID. (2013). MULTIPLEX PCR SCREENING OF Y-CHROMOSOME MICRODELETIONS IN AZOOSPERMIC ICSI CANDIDATE MEN. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 11(4), 335-338. SID. https://sid.ir/paper/296066/en

    Vancouver: Copy

    SHEIKHHA MOHAMMAD HASAN, ZAIMY MOHAMMAD ALI, SOLEIMANIAN SAEEDE, KALANTAR SEYED MEHDI, RASTI AZAM, GOLZADE MARYAM, HOSEINI FAHRAJI HAMID. MULTIPLEX PCR SCREENING OF Y-CHROMOSOME MICRODELETIONS IN AZOOSPERMIC ICSI CANDIDATE MEN. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2013;11(4):335-338. Available from: https://sid.ir/paper/296066/en

    IEEE: Copy

    MOHAMMAD HASAN SHEIKHHA, MOHAMMAD ALI ZAIMY, SAEEDE SOLEIMANIAN, SEYED MEHDI KALANTAR, AZAM RASTI, MARYAM GOLZADE, and HAMID HOSEINI FAHRAJI, “MULTIPLEX PCR SCREENING OF Y-CHROMOSOME MICRODELETIONS IN AZOOSPERMIC ICSI CANDIDATE MEN,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 11, no. 4, pp. 335–338, 2013, [Online]. Available: https://sid.ir/paper/296066/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button