The Effect of Monophosphoryl Lipid A on Maturation of DCs from Patients with Acute Myeloid Leukaemia

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El Husseiny Noha Mohamed; El Fattah Attya Elkak Walaa Abd; El Ansary Mervat Said; Abd El Aziz El Khateeb Engy Mohamed; Mattar Mervat Wagih; El Demerdash Doaa Mohamed

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Journal: IRANIAN JOURNAL OF IMMUNOLOGY Year:2017 | Volume:14 | Issue:1 Page(s): 1-12

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Title

The Effect of Monophosphoryl Lipid A on Maturation of DCs from Patients with Acute Myeloid Leukaemia

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DCs Maturation

Monocyte-Derived DCs

Abstract

Background: Generation of monocyte-derived dendritic cells (MDDC) is induced in the presence of GM-CSF and IL-4, and a maturation stimulus is added to the monocyte culture to obtain mature Dendritic Cells (DCs) suitable for therapy. TNF-α is the most common cytokine used for activating DCs and generating mature MDDC either alone or in combination with other cytokines. Objective: To compare effects of traditional cytokine cocktail (TNF-α + IL-1β ) versus TLR4-agonist monophosphoryl lipid A on the viability, phenotype, cytokine profile and functionality of MDDC. Methods: The study included 32 individuals; twenty Acute Myeloid Leukaemia (AML) cases in complete remission and 12 healthy volunteers. They were divided into 3 groups: Group 1: control group: 12 subjects to measure the baseline levels of all markers in the monocytic preparation; Group 2: cytokine cocktail (TNF-α ) group, which included 10 AML subjects; Group 3: MPLA group which included 10 AML subjects. Results: TNF-α group showed higher expression of CD83 than MPLA group indicating higher capacity to induce DC maturation but both were similar in CD86, CCR7 and IL-10 expression. Preparation of dendritic cells from AML cases in remission and loading them with tumor peptides was successful. Conclusion: The effect of MPLA in DC maturation is comparable with traditional DC maturation cocktail.

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APA: Copy

El Husseiny, Noha Mohamed, El Fattah Attya Elkak, Walaa Abd, El Ansary, Mervat Said, Abd El Aziz El Khateeb, Engy Mohamed, Mattar, Mervat Wagih, & El Demerdash, Doaa Mohamed. (2017). The Effect of Monophosphoryl Lipid A on Maturation of DCs from Patients with Acute Myeloid Leukaemia. IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 14(1), 1-12. SID. https://sid.ir/paper/297006/en

Vancouver: Copy

El Husseiny Noha Mohamed, El Fattah Attya Elkak Walaa Abd, El Ansary Mervat Said, Abd El Aziz El Khateeb Engy Mohamed, Mattar Mervat Wagih, El Demerdash Doaa Mohamed. The Effect of Monophosphoryl Lipid A on Maturation of DCs from Patients with Acute Myeloid Leukaemia. IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2017;14(1):1-12. Available from: https://sid.ir/paper/297006/en

IEEE: Copy

Noha Mohamed El Husseiny, Walaa Abd El Fattah Attya Elkak, Mervat Said El Ansary, Engy Mohamed Abd El Aziz El Khateeb, Mervat Wagih Mattar, and Doaa Mohamed El Demerdash, “The Effect of Monophosphoryl Lipid A on Maturation of DCs from Patients with Acute Myeloid Leukaemia,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 14, no. 1, pp. 1–12, 2017, [Online]. Available: https://sid.ir/paper/297006/en

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