STUDY OF THE EXOGENOUS INDUCTION OF OCT4 AND CONCOMITANT P53 INHIBITION ON THE EXPRESSION OF PLURIPOTENCY GENES IN HUMAN ADSCS

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RASHIDIPOUR ATEFEH; JAVERI ARASH; FAKHR TAHA MASOUMEH

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Journal: Daneshvar Medicine: Basic and Clinical Research Journal Year:2018 | Volume:25 | Issue:133 Page(s): 27-38

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Title

STUDY OF THE EXOGENOUS INDUCTION OF OCT4 AND CONCOMITANT P53 INHIBITION ON THE EXPRESSION OF PLURIPOTENCY GENES IN HUMAN ADSCS

Author(s)

RASHIDIPOUR ATEFEH | JAVERI ARASH | FAKHR TAHA MASOUMEH | Issue Writer Certificate

Keywords

ADIPOSE TISSUE-DERIVED STEM CELLSQ1

PLURIPOTENCYQ2

OCT4Q1

SHRNAQ1

P53 INHIBITIONQ1

Abstract

Background and Objective: OCT4 is the most important transcription factor for reprogramming of somatic cells and maintenance of PLURIPOTENCY. Moreover, it has recently been shown that loss of P53 or its mutations improve the efficiency of reprogramming. This study was designed to reprogram human ADIPOSE TISSUE-DERIVED STEM CELLS (ADSCs) by overexpression ofOCT4 and inhibition ofP53 expression.Materials and Methods: The ADSCs were isolated from abdominal fat tissue of patients who underwent cosmetic abdominoplasty. For characterization of the ADSCs, the expression of some mesenchymal stem cell markers was assessed by flow cytometry and the cells were differentiated into osteogenic and adipogenic lineages. The third-passaged ADSCs were transfected with a plasmid expressingOCT4 and a P53-shRNA. One week after transfection, the expression of PLURIPOTENCY genes was studied by quantitative real-time PCR.Results: The third-passaged ADSCs showed a fibroblast-like morphology.Meanwhile, 94%, 80.2%, and 81.1% of the ADSCs showed positive staining for CD90, CD73 and CD105 markers, respectively. Moreover, the ADSCs showed adipogenic and osteogenic differentiation. As revealed by qPCR analysis, the expression ofOCT4, SOX2, LIN28, REX1, CCND1 and C-MYC mRNAs were significantly upregulated after transfection of the ADSCs with hOCT4/shp53 vector compared to the control group.Conclusion: Overexpression of OCT4 and inhibition of P53 can improve reprogramming of the ADSCs toward a pluripotent state. This method may improve the differentiation capacity of the ADSCs for clinical applications.

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References

Impact ofearly subcultures on stemness, migration andangiogenic potential of adipose tissue-derivedstem cells and their resistance to in vitroischemic condition

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APA: Copy

RASHIDIPOUR, ATEFEH, JAVERI, ARASH, & FAKHR TAHA, MASOUMEH. (2018). STUDY OF THE EXOGENOUS INDUCTION OF OCT4 AND CONCOMITANT P53 INHIBITION ON THE EXPRESSION OF PLURIPOTENCY GENES IN HUMAN ADSCS. DANESHVAR MEDICINE, 25(133 ), 27-38. SID. https://sid.ir/paper/30435/en

Vancouver: Copy

RASHIDIPOUR ATEFEH, JAVERI ARASH, FAKHR TAHA MASOUMEH. STUDY OF THE EXOGENOUS INDUCTION OF OCT4 AND CONCOMITANT P53 INHIBITION ON THE EXPRESSION OF PLURIPOTENCY GENES IN HUMAN ADSCS. DANESHVAR MEDICINE[Internet]. 2018;25(133 ):27-38. Available from: https://sid.ir/paper/30435/en

IEEE: Copy

ATEFEH RASHIDIPOUR, ARASH JAVERI, and MASOUMEH FAKHR TAHA, “STUDY OF THE EXOGENOUS INDUCTION OF OCT4 AND CONCOMITANT P53 INHIBITION ON THE EXPRESSION OF PLURIPOTENCY GENES IN HUMAN ADSCS,” DANESHVAR MEDICINE, vol. 25, no. 133 , pp. 27–38, 2018, [Online]. Available: https://sid.ir/paper/30435/en

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