Evaluation of Enkephalin-Degrading Enzymes in Sperm from Heroin-Addicted Men

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Rezaei Mojaz Samira; NAZMARA ZOHREH; NAJAFI MOHAMMAD; MOVAHEDIN MANSOUREH; ZANDIEH ZAHRA; SHIRINBAYAN PEYMANEH; ROSHANPAJOUH MOHSEN; ASGARI HAMID REZA; ABBASI MEHDI; KORUJI MORTEZA

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Journal: International Journal of Fertility & Sterility Year:2020 | Volume:13 | Issue:4 Page(s): 301-306

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Title

Evaluation of Enkephalin-Degrading Enzymes in Sperm from Heroin-Addicted Men

Author(s)

Keywords

Addiction

Aminopeptidase N

Endopeptidase

Heroin

Sperm Quality

Abstract

Background: The aim of this study was to investigate two enkephalin-degrading enzymes, Aminopeptidase N (APN/ CD13) and Endopeptidase (NEP/CD10), gene and protein expression levels in sperm samples of fertile and Heroinaddicted men, and the correlation between their expressions and semen quality. Materials and Methods: In this case-controlled study, semen was collected from 24 normozoospermic healthy (as a control group) and 24 Heroin-addicted men donors (as case or Addiction group). Sperm cells isolated by Cook Medical gradient (40-80%) and followed up by swim-up techniques were used for real-time quantitative polymerase chain reaction (qPCR) and flow cytometry techniques to assess APN/CD13 and NEP/CD10 genes and proteins subsequently. Semen parameters were analyzed by computer-assisted sperm analysis. Results: The findings revealed that there were significant differences in sperm total motility (41. 07 ± 3. 63 vs. 63. 03 ± 3. 31 %, P=0. 0001), progressive motility (35. 21 ± 2. 64 vs. 20. 93 ± 3. 22%, P=0. 001) and viability (69. 9 ± 4. 69 vs. 86. 81 ± 1. 26 %, P=0. 002) in the addicted group vs. control ones. APN and NEP gene expression levels in the addicted group decreased compared with the control ones (1. 00 ± 0. 67 vs. 0. 36 ± 0. 13, P= 0. 008 and 1. 07 ± 0. 11 vs. 0. 52 ± 0. 12 0. 002, respectively). Flow cytometry analysis showed that the average percent of APN/CD13 in Heroin consumers significantly decreased compared with the healthy ones, while NEP/CD10 rate between two groups was similar. We also observed that duration of drug dependence is correlated with sperm viability (r=-0. 627, P=0. 016) and motility (r=-0. 410, P=0. 05), NEP (r=-0. 434, P= 0. 049), and APN (r=-0. 641, P=0. 002) gene expression levels. Conclusion: We conclude that semen quality and enkephalin-degrading enzymes were altered in Heroin-addicted men. other confirming the internal validity of our estimates.

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The Psychometric Properties of the Drug Abuse Screening Test

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APA: Copy

Rezaei Mojaz, Samira, NAZMARA, ZOHREH, NAJAFI, MOHAMMAD, MOVAHEDIN, MANSOUREH, ZANDIEH, ZAHRA, SHIRINBAYAN, PEYMANEH, ROSHANPAJOUH, MOHSEN, ASGARI, HAMID REZA, ABBASI, MEHDI, & KORUJI, MORTEZA. (2020). Evaluation of Enkephalin-Degrading Enzymes in Sperm from Heroin-Addicted Men. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 13(4), 301-306. SID. https://sid.ir/paper/306070/en

Vancouver: Copy

Rezaei Mojaz Samira, NAZMARA ZOHREH, NAJAFI MOHAMMAD, MOVAHEDIN MANSOUREH, ZANDIEH ZAHRA, SHIRINBAYAN PEYMANEH, ROSHANPAJOUH MOHSEN, ASGARI HAMID REZA, ABBASI MEHDI, KORUJI MORTEZA. Evaluation of Enkephalin-Degrading Enzymes in Sperm from Heroin-Addicted Men. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2020;13(4):301-306. Available from: https://sid.ir/paper/306070/en

IEEE: Copy

Samira Rezaei Mojaz, ZOHREH NAZMARA, MOHAMMAD NAJAFI, MANSOUREH MOVAHEDIN, ZAHRA ZANDIEH, PEYMANEH SHIRINBAYAN, MOHSEN ROSHANPAJOUH, HAMID REZA ASGARI, MEHDI ABBASI, and MORTEZA KORUJI, “Evaluation of Enkephalin-Degrading Enzymes in Sperm from Heroin-Addicted Men,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 13, no. 4, pp. 301–306, 2020, [Online]. Available: https://sid.ir/paper/306070/en

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