DEVELOPMENT OF ELISA METOHOD TO ASSAY SERUM GLUTATHIONE - S-TRANSFERASE -Π IN PATIENTS
CANCEROUS

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ZAREEI MAHMOUDABADI A.B.; RASAEI M.J.; ALAMEH A.A.; SHIRBAZOU SH.

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Journal Paper

Paper Information

Journal: Daneshvar Medicine: Basic and Clinical Research Journal Year:2003 | Volume:10 | Issue:45 Page(s): 49-56

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Title

DEVELOPMENT OF ELISA METOHOD TO ASSAY SERUM GLUTATHIONE - S-TRANSFERASE -Π IN PATIENTS CANCEROUS

Author(s)

ZAREEI MAHMOUDABADI A.B. | RASAEI M.J. | ALAMEH A.A. | SHIRBAZOU SH. | Issue Writer Certificate

Keywords

ELISAQ2

GST-ΠQ4

CANCERQ4

Abstract

In this study, an indirect ELISA method was developed by using the Horse Radish peroxidase enzyme (HRP) and GST-Π which purified from human placenta first using the purified GST- π polycolonal antibody was produced and the stages of designing method were carried out by contribution of secondary antibody conjugated with peroxidase. This method was standardized by using the standard GST- π in buffer medium and in a serum sample which immunostripped from endogenous GST- π. using this method, some concentrations between 0.4 to 20 ng/ml from serum GST- π are measurable without cross reaction with other isoenzymes of GST (µ and α). The specificity of this method is to investigate, using of other isoenzyes such as GST- µ and GST- α which purified from rat liver, that their interference was maximum 5.8% in limited working concentration range. The accuracy of the system was studied by addition of three different concentration of exogenouse GST- π to immunostripped serum, the recovery rate in different concentrations was between 89-106%. Inter and intra assay variations showed a CV less than 12%. Then, using the developed ELISA and GST- π were measured in the serum of 42 healthy controls and quantities over 16 ng/ml were observed in 90% of patients who suffering from stomach CANCER and 86% patients suffering from esophagous CANCER.

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APA: Copy

ZAREEI MAHMOUDABADI, A.B., RASAEI, M.J., ALAMEH, A.A., & SHIRBAZOU, SH.. (2003). DEVELOPMENT OF ELISA METOHOD TO ASSAY SERUM GLUTATHIONE - S-TRANSFERASE -Π IN PATIENTS CANCEROUS. DANESHVAR MEDICINE, 10(45), 49-56. SID. https://sid.ir/paper/30648/en

Vancouver: Copy

ZAREEI MAHMOUDABADI A.B., RASAEI M.J., ALAMEH A.A., SHIRBAZOU SH.. DEVELOPMENT OF ELISA METOHOD TO ASSAY SERUM GLUTATHIONE - S-TRANSFERASE -Π IN PATIENTS CANCEROUS. DANESHVAR MEDICINE[Internet]. 2003;10(45):49-56. Available from: https://sid.ir/paper/30648/en

IEEE: Copy

A.B. ZAREEI MAHMOUDABADI, M.J. RASAEI, A.A. ALAMEH, and SH. SHIRBAZOU, “DEVELOPMENT OF ELISA METOHOD TO ASSAY SERUM GLUTATHIONE - S-TRANSFERASE -Π IN PATIENTS CANCEROUS,” DANESHVAR MEDICINE, vol. 10, no. 45, pp. 49–56, 2003, [Online]. Available: https://sid.ir/paper/30648/en

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