EVALUATION OF MULTIPLEX NESTED POLYMERASE CHAIN REACTION FOR ROUTINE HEPATITIS C VIRUS GENOTYPING IN EGYPTIAN PATIENTS

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SHEMIS MOHAMED ABBAS; MOHAMED EL ABD DINA; IBRAHIM RAMADAN DALIA; IBRAHIM EL SAYED MOHAMED; SHENODA GUIRGIS BASSEM; SABER MOHAMED ALI; EL SAID AZZAZY HASSAN MOHAMED

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Journal: Hepatitis Monthly Year:2012 | Volume:12 | Issue:4 Page(s): 265-270

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Title

EVALUATION OF MULTIPLEX NESTED POLYMERASE CHAIN REACTION FOR ROUTINE HEPATITIS C VIRUS GENOTYPING IN EGYPTIAN PATIENTS

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Keywords

HEPATITIS C

MULTIPLEX POLYMERASE CHAIN REACTION

BRANCHED DNA SIGNAL AMPLIFICATION

ASSAY

Abstract

Background: At least six HCV (HEPATITIS C virus) genotypes are unequally distributed worldwide. HCV genotyping guides the selection of treatment regimens and provides important epidemiological markers that enable the outbreak source to be traced and the spread of disease to be controlled. In Egypt, there is an increasing need for cost-effective, fast, and easily performable HCV genotyping ASSAYs.Recently, a multiplex PCR ASSAY was developed to determine HCV genotypes. It employs genotype-specific primers, based on sequences of the entire core region and part of the 5’UTR of the genome.Objectives: In this study, we compared a simple, new, modified multiplex PCR system for HCV genotyping with a commercially available line probe ASSAY (INNO-LiPA) that is based on reverse hybridization.Patients and Methods: Serum samples from chronic HCV Egyptian patients (n=73) were genotyped using the modified multiplex PCR ASSAY, and genotypes were verified using the INNO-LiPA HCV II ASSAY.Results: The modified multiplex PCR method was able to type HCV-4 in 65 of 70 typeable samples (92.86%) and had 100% concordance with the INNO-LiPA ASSAY.Conclusions: Genotype 4 was the most prevalent genotype in our study. Based on our results, the modified multiplex nested PCR ASSAY is a sensitive and inexpensive alternative for HCV genotyping and can be used in routine diagnostic laboratories. INNO-LiPA may be useful as a second-line ASSAY for genotyping samples that are indeterminate by multiplex PCR. This approach will effect better treatment optimization and a reduction of the spread of HCV.

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APA: Copy

SHEMIS, MOHAMED ABBAS, MOHAMED EL ABD, DINA, IBRAHIM RAMADAN, DALIA, IBRAHIM EL SAYED, MOHAMED, SHENODA GUIRGIS, BASSEM, SABER, MOHAMED ALI, & EL SAID AZZAZY, HASSAN MOHAMED. (2012). EVALUATION OF MULTIPLEX NESTED POLYMERASE CHAIN REACTION FOR ROUTINE HEPATITIS C VIRUS GENOTYPING IN EGYPTIAN PATIENTS. HEPATITIS MONTHLY, 12(4), 265-270. SID. https://sid.ir/paper/306543/en

Vancouver: Copy

SHEMIS MOHAMED ABBAS, MOHAMED EL ABD DINA, IBRAHIM RAMADAN DALIA, IBRAHIM EL SAYED MOHAMED, SHENODA GUIRGIS BASSEM, SABER MOHAMED ALI, EL SAID AZZAZY HASSAN MOHAMED. EVALUATION OF MULTIPLEX NESTED POLYMERASE CHAIN REACTION FOR ROUTINE HEPATITIS C VIRUS GENOTYPING IN EGYPTIAN PATIENTS. HEPATITIS MONTHLY[Internet]. 2012;12(4):265-270. Available from: https://sid.ir/paper/306543/en

IEEE: Copy

MOHAMED ABBAS SHEMIS, DINA MOHAMED EL ABD, DALIA IBRAHIM RAMADAN, MOHAMED IBRAHIM EL SAYED, BASSEM SHENODA GUIRGIS, MOHAMED ALI SABER, and HASSAN MOHAMED EL SAID AZZAZY, “EVALUATION OF MULTIPLEX NESTED POLYMERASE CHAIN REACTION FOR ROUTINE HEPATITIS C VIRUS GENOTYPING IN EGYPTIAN PATIENTS,” HEPATITIS MONTHLY, vol. 12, no. 4, pp. 265–270, 2012, [Online]. Available: https://sid.ir/paper/306543/en

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