A COMPARISON STUDY OF MICROBIOLOGIC AND SEROLOGIC DIAGNOSTIC METHODS FOR HELICOBACTER PYLORI INFECTION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HOUDEHEI E.; SARAFNEZHAD A.A.F.; SIAVASHI FARIDEH; MASERAT S.; EHSANI ARDAKANI M.J.; JADALI Z.; SHAHRESTANI T.; HASHEMI S.M.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: DANESHVAR MEDICINE Year:2003 | Volume:11 | Issue:47 Page(s): 73-80

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,208

Download:

Cites:

Information Journal Paper

Title

A COMPARISON STUDY OF MICROBIOLOGIC AND SEROLOGIC DIAGNOSTIC METHODS FOR HELICOBACTER PYLORI INFECTION

Author(s)

Keywords

HELICOBACTER PYLORIQ3

ELISAQ4

IFAQ4

UREASE TESTQ4

CULTUREQ4

DIRECT SMEAR & GASTRODUODENAL DISEASESQ4

Abstract

According to the role of HELICOBACTER PYLORI (H. Pylori) in induction of gastroduodenal diseases, and its high prevalence in Iran, it is important to have an available sensitive and easily used diagnostic method. Common methods used for H. pylori diagnosis are based on invasive and non invasive techniques. Among non invasive procedures, serologic tests are convenient choice. Here we evaluated the efficiency of ELISA and Indirect Immuno Fluorescence Assay (IFA) for H. Pylori diagnosis. Crude antigen, extracted from bacterial isolates of Iranian patients, was used for ELISA and intact bacteria were used in IFA.175 patients suffering 1from gastroduodenal diseases, who were referred to Shariati and Taleghani Hospitals in Tehran, were included. From each patient, two biopsies and 5 ml blood samples were collected.Rapid UREASE TEST, CULTURE and direct smear with gram staining were performed on biopsies. These tests used as a gold standard. ELISA and IFA which were previously standardized were carried out on patient's serum.The sensitivity and specificity for ELISA were 93.2% and 95.4%, and for IFA method were 98.6% and 72.7% respectively. In addition the sensitivity of rapid UREASE TEST, CULTURE and direct smear were 84.3%, 85%, 91.2% respectively.However, microbiologic methods have high specificity but each of them shows reduced sensitivity compared to serologic tests. Serologic methods are economical, easy and safety, to perform; therefore they can be employed in diagnois of H. pylori infection and also in epidemiological studies.

Cites

No record.

References

Cite

APA: Copy

HOUDEHEI, E., SARAFNEZHAD, A.A.F., SIAVASHI, FARIDEH, MASERAT, S., EHSANI ARDAKANI, M.J., JADALI, Z., SHAHRESTANI, T., & HASHEMI, S.M.. (2003). A COMPARISON STUDY OF MICROBIOLOGIC AND SEROLOGIC DIAGNOSTIC METHODS FOR HELICOBACTER PYLORI INFECTION. DANESHVAR MEDICINE, 11(47), 73-80. SID. https://sid.ir/paper/30658/en

Vancouver: Copy

HOUDEHEI E., SARAFNEZHAD A.A.F., SIAVASHI FARIDEH, MASERAT S., EHSANI ARDAKANI M.J., JADALI Z., SHAHRESTANI T., HASHEMI S.M.. A COMPARISON STUDY OF MICROBIOLOGIC AND SEROLOGIC DIAGNOSTIC METHODS FOR HELICOBACTER PYLORI INFECTION. DANESHVAR MEDICINE[Internet]. 2003;11(47):73-80. Available from: https://sid.ir/paper/30658/en

IEEE: Copy

E. HOUDEHEI, A.A.F. SARAFNEZHAD, FARIDEH SIAVASHI, S. MASERAT, M.J. EHSANI ARDAKANI, Z. JADALI, T. SHAHRESTANI, and S.M. HASHEMI, “A COMPARISON STUDY OF MICROBIOLOGIC AND SEROLOGIC DIAGNOSTIC METHODS FOR HELICOBACTER PYLORI INFECTION,” DANESHVAR MEDICINE, vol. 11, no. 47, pp. 73–80, 2003, [Online]. Available: https://sid.ir/paper/30658/en

Related Journal Papers