COMPARISON BETWEEN IFA AND ELISA TECHNIQUE WITH PCR METHOD IN THE DIAGNOSIS OF TOXOPLASMA GONDI PARASITE IN INFANTS ADMITTED AT TALEGHANI HOSPITAL

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KARAMI M.; MAHBOD S.A.A.; SHAD DEL M.

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Journal: Annals of Military and Health Sciences Research Year:2007 | Volume:5 | Issue:1 (SERIAL NUMBER 17) Page(s): 1141-1149

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Title

COMPARISON BETWEEN IFA AND ELISA TECHNIQUE WITH PCR METHOD IN THE DIAGNOSIS OF TOXOPLASMA GONDI PARASITE IN INFANTS ADMITTED AT TALEGHANI HOSPITAL

Author(s)

KARAMI M. | MAHBOD S.A.A. | SHAD DEL M. | Issue Writer Certificate

Keywords

ELISAQ3

IFAQ3

TOXOPLASMA GONDIQ4

Abstract

Background: There are some methods to detect toxoplasma in infected patients. In this survey we compared sensitivity of two methods of IFA & ELISA.Material & methods: In this survey the sera of 106 newborn infants from 1 day to 1 month age who were suspected to congenital toxoplasmosis and were hospitalized in infants' ward of Taleghani hospital were evaluated for IgM and IgG specific antibodies with ELISA and IFA techniques respectively. At the same time blood/CS For both of them were evaluated for genome of this parasite by PCR techniques in 115 suspected infants.Results: Results of PCR were positive in 6 infants (%5.22) which suggested congenital Toxoplasmosis. The 1/100 titer of IgM specific antibodies was positive in 5 or them by IFA technique. If we suggest that PCR is gold standard technique for Toxoplasmosis diagnosis so the sensitivity of 1/100 titer of IgM antibodies by IFA technique would be 83.3 percent and because no other infants had a positive result so the specificity of this test would be 100 percent.Conclusion: All 6 infected infants had a positive result from ELISA technique for IgM specific antibodies so the sensitivity of this test was 100 percent. Because no other infants had a positive result, so the specificity of this test was 100 percent. 41 infants had 1/200 titer of specific IgG antibodies by IFA technique. With deletion of 6 infected infants, the prevalence of chronic toxoplasmosis between the mothers whose infants was confined in Taleghani hospital was 34 Percent. This prevalence was 30.2 percent by ELISA technique. Therefore IFA method is more sensitive than ELISA method in detection of TOXOPLASMA GONDI.

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APA: Copy

KARAMI, M., MAHBOD, S.A.A., & SHAD DEL, M.. (2007). COMPARISON BETWEEN IFA AND ELISA TECHNIQUE WITH PCR METHOD IN THE DIAGNOSIS OF TOXOPLASMA GONDI PARASITE IN INFANTS ADMITTED AT TALEGHANI HOSPITAL. ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH, 5(1 (SERIAL NUMBER 17)), 1141-1149. SID. https://sid.ir/paper/96502/en

Vancouver: Copy

KARAMI M., MAHBOD S.A.A., SHAD DEL M.. COMPARISON BETWEEN IFA AND ELISA TECHNIQUE WITH PCR METHOD IN THE DIAGNOSIS OF TOXOPLASMA GONDI PARASITE IN INFANTS ADMITTED AT TALEGHANI HOSPITAL. ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH[Internet]. 2007;5(1 (SERIAL NUMBER 17)):1141-1149. Available from: https://sid.ir/paper/96502/en

IEEE: Copy

M. KARAMI, S.A.A. MAHBOD, and M. SHAD DEL, “COMPARISON BETWEEN IFA AND ELISA TECHNIQUE WITH PCR METHOD IN THE DIAGNOSIS OF TOXOPLASMA GONDI PARASITE IN INFANTS ADMITTED AT TALEGHANI HOSPITAL,” ANNALS OF MILITARY AND HEALTH SCIENCES RESEARCH, vol. 5, no. 1 (SERIAL NUMBER 17), pp. 1141–1149, 2007, [Online]. Available: https://sid.ir/paper/96502/en

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