HEPATITIS C VIRUS CAPSID PROTEIN AND INTRACELLULAR LIPIDS INTERPLAY AND ITS ASSOCIATION WITH HEPATIC STEATOSIS

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AFZAL MUHAMMAD SOHAIL; SADAF ZAIDI NAJAM US SAHAR; DUBUISSON JEAN; ROUILLE YVES

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Journal Paper

Paper Information

Journal: Hepatitis Monthly Year:2014 | Volume:14 | Issue:8 Page(s): 0-0

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Title

HEPATITIS C VIRUS CAPSID PROTEIN AND INTRACELLULAR LIPIDS INTERPLAY AND ITS ASSOCIATION WITH HEPATIC STEATOSIS

Author(s)

AFZAL MUHAMMAD SOHAIL | SADAF ZAIDI NAJAM US SAHAR | DUBUISSON JEAN | ROUILLE YVES | Issue Writer Certificate

Keywords

HEPATITIS C VIRUS

FATTY LIVER

INTRACELLULAR LIPIDS

STEATOSIS

Abstract

Background: HEPATITIS C VIRUS (HCV) is a major causative agent for chronic liver disease worldwide. Hepatic STEATOSIS is a frequent histological feature in patients with chronic HCV. Both host and viral factors are involved in STEATOSIS development. It results from uncontrolled growth of cytoplasmic lipid droplets (LDs) in hepatocytes. LDs are intracellular organelles playing key role in the HCV life cycle. HCV core protein localizes at the LD surface and this localization is crucial for virion production. Objectives: We explored in vitro interplay of core and LDs to investigate the role of core in STEATOSIS.Materials and Methods: Core expression vectors were transfected in Huh-7 cells. The effect of core protein on LDs content and distribution in the cells was monitored by confocal microscopy. Cells were treated with oleic acid to analyze the effect of increased intracellular LDs on core expression. Core protein expression was monitored by western blot analysis.Results: Core expression altered the intracellular lipid metabolism, which resulted in a change in LDs morphology. Core LDs interaction was required for this effect since the mutation of two prolines (P138A, P143A), which impair LDs localization, had no impact on LDs morphology. Conversely, oleic acid induced intracellular LD content resulted in increased core expression.Conclusions: Core-LDs interaction may be an underlying molecular mechanism to induce liver STEATOSIS in patients with HCV infection. This interaction is also crucial for efficient viral replication and persistence in infected cells. STEATOSIS can also interfere with efficient standard interferon therapy treatment. Management of STEATOSIS should be considered along with standard care for achieving higher sustained virological response (SVR) in patients receiving interferon regimen.

Cites

HETEROLOGOUS EXPRESSION OF HEPATITIS C VIRUS CORE PROTEIN IN OIL SEEDS OF BRASSICA NAPUS L.

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APA: Copy

AFZAL, MUHAMMAD SOHAIL, SADAF ZAIDI, NAJAM US SAHAR, DUBUISSON, JEAN, & ROUILLE, YVES. (2014). HEPATITIS C VIRUS CAPSID PROTEIN AND INTRACELLULAR LIPIDS INTERPLAY AND ITS ASSOCIATION WITH HEPATIC STEATOSIS. HEPATITIS MONTHLY, 14(8), 0-0. SID. https://sid.ir/paper/306633/en

Vancouver: Copy

AFZAL MUHAMMAD SOHAIL, SADAF ZAIDI NAJAM US SAHAR, DUBUISSON JEAN, ROUILLE YVES. HEPATITIS C VIRUS CAPSID PROTEIN AND INTRACELLULAR LIPIDS INTERPLAY AND ITS ASSOCIATION WITH HEPATIC STEATOSIS. HEPATITIS MONTHLY[Internet]. 2014;14(8):0-0. Available from: https://sid.ir/paper/306633/en

IEEE: Copy

MUHAMMAD SOHAIL AFZAL, NAJAM US SAHAR SADAF ZAIDI, JEAN DUBUISSON, and YVES ROUILLE, “HEPATITIS C VIRUS CAPSID PROTEIN AND INTRACELLULAR LIPIDS INTERPLAY AND ITS ASSOCIATION WITH HEPATIC STEATOSIS,” HEPATITIS MONTHLY, vol. 14, no. 8, pp. 0–0, 2014, [Online]. Available: https://sid.ir/paper/306633/en

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