HUMAN MONOPOSAL GONADOTROPIN STIMULATION OF FOLLICLES IN GRAFTED VITRIFIED MOUSE OVARIES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SALEHNIA M.; MOAZENI S.M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: DANESHVAR MEDICINE Year:2002 | Volume:9 | Issue:38 Page(s): 25-32

Download Full-Text

Persian Verion

View:

838

Download:

Cites:

Information Journal Paper

Title

HUMAN MONOPOSAL GONADOTROPIN STIMULATION OF FOLLICLES IN GRAFTED VITRIFIED MOUSE OVARIES

Author(s)

SALEHNIA M. | MOAZENI S.M. | Issue Writer Certificate

Keywords

VITRIFICATIONQ4

OVARIAN AUTOGRAFT AND HORMONAL STIMULATIONQ4

HUMAN MONOPOSAL GONADOTROPINQ4

Abstract

The aim of this study was to investigate the effects of daily injection of hMG on the development of follicles in transplanted vitrified mouse ovary to improve the in vivo growth of primordial and primary follicles. Ovaries from 8-10 weeks age and NMRI mice were vitrified by using a solution of RPMI containing 30% ((W/V) follicles 70, 0.5 M sucrose, 10.7% (V/V) acetamid and 40% (V/V) ethylene glycol (EGFS40%) and stored in liquid nirtogen. After thawing by I M sucrose solution and equilibration with RPIM medium, the frozen-thawed and fresh ovarian tissues were auto grafted intraperitonealy. One day after transplantation, stimulation was started by injection of 5 IU hMG (Personal 500, Serono) in one group of grafted mouse each other day for 9 days. Stimulated and non-stimulated animals were sacrificed during 3, 5, 7, 9 and 11 days after hMG injection. The vitrified and non-vitrified grafts and fresh tissue were fixed in Bouin’s fixative and processed for histology. In the fresh control ovaries almost all of the follicles appeared normal in the various development stages. In vitrified ovaries there were no morphologically differences between transplanted tissue with or without hormonal injection. Three days after passing the transplantation, a lot of large prenatal and antral follicles were degenerated but after 10 days just normal primordial and primary follicles were observed, the stroma was devoid the necrotic cells. The grafted ovarian tissues were invaded by a lot of fat and fibrous cells. Thus VITRIFICATION procedure is a useful and efficient procedure for cry preservation of ovarian tissues, however it is necessary to improve the maturation of primordial and primary follicles by changing the site of transplantation or hormonal stimulation with effective dosages.

Cites

No record.

References

ULTRASTRUCTURE OF PRIMARY FOLLICLE AFTER VITRIFICATION OF MOUSE OVARY

Cite

APA: Copy

SALEHNIA, M., & MOAZENI, S.M.. (2002). HUMAN MONOPOSAL GONADOTROPIN STIMULATION OF FOLLICLES IN GRAFTED VITRIFIED MOUSE OVARIES . DANESHVAR MEDICINE, 9(38), 25-32. SID. https://sid.ir/paper/30820/en

Vancouver: Copy

SALEHNIA M., MOAZENI S.M.. HUMAN MONOPOSAL GONADOTROPIN STIMULATION OF FOLLICLES IN GRAFTED VITRIFIED MOUSE OVARIES . DANESHVAR MEDICINE[Internet]. 2002;9(38):25-32. Available from: https://sid.ir/paper/30820/en

IEEE: Copy

M. SALEHNIA, and S.M. MOAZENI, “HUMAN MONOPOSAL GONADOTROPIN STIMULATION OF FOLLICLES IN GRAFTED VITRIFIED MOUSE OVARIES ,” DANESHVAR MEDICINE, vol. 9, no. 38, pp. 25–32, 2002, [Online]. Available: https://sid.ir/paper/30820/en

Related Journal Papers