Daneshvar Medicine: Basic and Clinical Research Journal
Year:2002 | Volume:9 | Issue:38|Papers Count:10

Anaerobic bacteria has an important role in periodontitis and Porphyromonas gingivalis is introduced as a major agent. Transport of anaerobic samples to laboratory is the first step and a very critical subject in quantitative study. Thioglycollate is usually used as a transport medium for anaerobic bacteria in Iran. In this study, we investigated the effects of time (0,1,3, and 5h) and supplements (Vit. K1, Hemin and Sodium bicarbonate) on the numbers of inoculated P. gingivalis. The results showed that just in the first hour after sampling in the thioglycollate (without supplement) the numbers of inoculated bacteria didn’t show significant reduction, but other conditions, the numbers of inoculated bacteria seriously reduced.The results suggest that we must use a better medium for transport of fastidious anaerobic bacteria.

Hepatolenticular degeneration (Wilson disease) is a hereditary disease in which Metabolic disorder of copper leads to the accumulation of Cu in the liver, brain, cornea and kidneys with consequent of pathologic changes in them. Hereditary mechanism of the disease is an autosomal recessive with prevalence of 1-100 per 1,000,000 inhabitants. This is a descriptive and retrospective study in which 46 patients suffering from wilson disease were diagnosed and studied during 3 years in Namazi Hospital, in Shiraz. The results show that 82% of patients had significant signs and symptoms and the average age was ±92.93 years (4-16). The most common symptom was abdominal protrusion (84.2%), 29(76%) had Jaundice, 28(74%) had As cites, 70% had Kaiser Fleisher ring. Abnormal abdominal examination was seen in 44 patients (45.5%) and abnormal Neurogical examination was seen in 19 patients (31.3%). Most of the patients had prolong PT, PTT, 32(70%) Hb<10 had. Normal U/A was seen in 17(36.5%) and most common abnormality, was hematuria in U/A. Abnormal LFT was seen in most patients. Fulminanat hepatitis was seen in 7 patients that all died from it. Our findings were similar to the other researches and dtudies. Finally we recommend the prevention of a new born child in family in which the child will born with wilson disease and also recommend that a child with jaundice (especially at age of 5-15 years old) suspection to wilson diseases is necessary. Siblings of the patients with wilson disease will be investigated to rule out wilson disease

It should be explored the stressful origins, considering the influence of stress on nurse work, so that confronting with those stresses, in order to in crease the competence, increase the strength of confronting with stressful factors and if possible decrease the environmental stressful factors. This research is analysis of longitudinal kind which has been done on 99 nurses by the help of questionnaire in 1999 in order to determine the stressful origins on nurses environment. 7.67% of nurses are female and 32.3% are male, 96% are expert with the average of 31 years old and average years of service is 6.82.7.69% of nurses are married, 38.4% have child, 26.3% are in surgery ward, 22.2% are in special wards and 48.5% work in the rest wards. Most of nurses mentioned that stressful factors are injustice by the authorities and the most desirable coping method among the nurses in confronting with stressful factors is hope to the future to improve the condition and the least method is usage of antianxioty. The relation of demographic variables with stressful factors and coping methods are studied so that there has not been distinguished any statistical meaningful relation (P=0.242). By recognizing of these factors, one should program and fulfill methods for logical coping with stressful origins of nurses environment.

The aim of this study was to investigate the effects of daily injection of hMG on the development of follicles in transplanted vitrified mouse ovary to improve the in vivo growth of primordial and primary follicles. Ovaries from 8-10 weeks age and NMRI mice were vitrified by using a solution of RPMI containing 30% ((W/V) follicles 70, 0.5 M sucrose, 10.7% (V/V) acetamid and 40% (V/V) ethylene glycol (EGFS40%) and stored in liquid nirtogen. After thawing by I M sucrose solution and equilibration with RPIM medium, the frozen-thawed and fresh ovarian tissues were auto grafted intraperitonealy. One day after transplantation, stimulation was started by injection of 5 IU hMG (Personal 500, Serono) in one group of grafted mouse each other day for 9 days. Stimulated and non-stimulated animals were sacrificed during 3, 5, 7, 9 and 11 days after hMG injection. The vitrified and non-vitrified grafts and fresh tissue were fixed in Bouin’s fixative and processed for histology. In the fresh control ovaries almost all of the follicles appeared normal in the various development stages. In vitrified ovaries there were no morphologically differences between transplanted tissue with or without hormonal injection. Three days after passing the transplantation, a lot of large prenatal and antral follicles were degenerated but after 10 days just normal primordial and primary follicles were observed, the stroma was devoid the necrotic cells. The grafted ovarian tissues were invaded by a lot of fat and fibrous cells. Thus vitrification procedure is a useful and efficient procedure for cry preservation of ovarian tissues, however it is necessary to improve the maturation of primordial and primary follicles by changing the site of transplantation or hormonal stimulation with effective dosages.

Chronic illness is a stressor factor that effects on family. So patient and his family must learn how to live with the disease and cope with it. In this descriptive study, based on pilot study and method based on objective, 62 diabetic children (7-12 years old, having at least one year duration diabetes, without other chronic or acute physical-emotional illness) were selected from two treatment centers in Tehran city. Considering the attendance of children and their parents in education courses of diabetes control, coping methods were assessed by question are. The result showed that 48.38% of children and their family or their replacement were present in aforesaid education. Survey of five coping methods (Develope competence and optimism, comply with treatment, seek support, feel different and withdrawal, irritable moody and acts out) showed non effective use in seek support (35.5%) and irritable moody and acts out methods (12.9%) more than other methods. Also the findings showed that attendance of child with parents in education classes caused to decrease non effective use of irritable moody and acts out method (p=0.006), and increase the effective use of comply with education method (p=0.01). Other aspects of coping methods with education of a child and parent had statistically no significant relation.

Purpose: Most spinal cord injured patients require accessory equipments for ejaculation which one of them is Electro ejaculation. Unfortunately, despite the sufficient number and volume of sperm, there is disorder in quality of sperm. In the article sperm motility, one of the quality disorders, has been evaluated. Material and Methods: In this research, the results of spermogram of spinal cord injured patients due to war were evaluated during the years of 1993-1998 in Kosar Infertility Center. Result: Not only the percentage of motile sperm but also the quality of motility (high grade motility) was lower than normal limit.Conclusion: Sperms were obtained by Electro ejaculation in patients with spinal cord injury, have not satisfactory motility which could be one reason of low fertility rate in these patients and require more study to find the cause.

Survey in the field of Iranian, Indian and Chinese medicine customy indicate that most former and recent medicine customy philosophers understand the therapeutic benefits of consumption of leaves and stems of Viscum album and administer them extensively the treatment of many disorders. However the therapeutic effects of Viscum album fruit (mistletoe) is not enough evaluated. The present research is designed to study the anti- diabetic effects of mistletoe aqueous extract.By subcutaneous injection of Alloxan (160mg/kg body weight) 14 male Wistar rats were become diabetic and then randomly divided into experimental and control groups (n=7). During the experimental period, the experimental rats were received aqueous extract of mistletoe (0.5 g/lit) through drinking water. Mistletoes were collected from Mazandaran forests in north of Iran. Blood samples were collected weekly from rats of both groups and the blood pH, the glucose, urea, triglyceride and cholesterol levels of blood serum and aceto-acetic acid level of urine were measured. The symptoms of diabetes mellitus disease include metabolic alterations which preliminary recognized by hyperglycemia and glycosuria and then by disorders of protein and fat metabolism, followed by body weight lose, alteration of blood pH (acidosis) and other biochemical factors in internal environment. The obtaining results of the present research indicate the obvious anti- diabetic effects of mistletoe consumption. Because Alloxan destroys Beta cells of probably by increasing the permeability of cells plasma membrane to glucose, prevents the progressive disorders and corrects the metabolic alterations due to diabetes.

Experimental study which has been done in the Orthopedic wards of Mobasher Kashani hospital inن Present study is an quazi Hamadan. The Purpose of the study was to identify the effect of representational structures on pain after musculo- skeletal injury in school age children who hospitalized in trauma center. In this study 75 patients (two groups of case and control) have taken part. The tools of assessment consist of behavioral and physological signs checklist and Oucher self report scale. In case and control groups before interaction, the rate of pain has been evaluated by 3 mentioned tools then the pain rate of interaction in both groups which includes watching comic films by video and television have been measured. Statistical analysis of comparison of the behavioral, psychological sings and Oucher scale before and after interaction in both case and control groups "pair t test" have been used and in order to determine the reduction of mentioned indicators, in these groups "t Test" was used. According to the result in control group before and after interaction there isn"t any meaningful difference in Oucher, behavioral and psychological sings score (P>0.05). While in case group before and after interaction, there is a meaningful differnce in mentioned indicators (P<0.05). Also non pair t test shows that in comparison between these two groups, case group in comparison with control group, there is a significant reduction in self report Ocucher, behavior signs score, pulse rate and systolic blood pressure (p=0). Therefore it can be used of comic representational structures to decrease the pain in injured children. The study also shows that there is a significant correlation between pain indicators (Oucher scale, behavioral and psychological signs).

Norplant implant system is a long term hormonal and reversible method of contraception which consisting of six flexible capsules are placed subdermally in the inner layer of the non dominant arm and provides protection for pregnancy up to five .1993years. In a historical cohort study, 2103 Norplant acceptors in the research center of family planning of Gilan from Dec Rank test, and the effects of to Dec 1999 were studied and the results were analyzed by Kaplan Meier method and Log variables with using Cox Regression model were analyzed. The continuation rate of Norplant at first year and the end of fifth year were 97% and 63% respectively. The removal rate before 5 years was 27.2% and menstrual disturbances was the most common cause of the removal (62.18%). The mean duration of using was 39.75 months. There wasn’t any Norplant failure during the study. There wasn’t any statistical relation between age, parity, education and occupation with continuation rate of Norplant. There was a significant statistical relation between previous contraception method and continuation rate. DMPA users had the most and IUD users had the lowest continuation rate. The continuation rate in cases, who had irregular menses before Norplant insertion, was lower than who had regular menses (P<0.007).

The present study has been done in order to survey the knowledge, attitude and practice of health workers of Iran University of Medical Sciences regarding the prevention of cervical cancer.experimental research which has been done on 125 health workers. 45 health workers from Shahryar andن This study is a semi Robatkarim constitute the case group. The control group consists of 80 health workers from Karaj and Savojbolagh.Before the instruction, there were no significant differences between the case and control group regarding demographic variables (age, education, work experience, previous instruction, ...) and their K.A.P. about cervical cancers and its prevention. The data was collected through structured questionnaires, observation, and checklists. By the way, family health files were studied before and after the instruction. The instructions were presented through lectures and by using films, booklets, pamphlets and etc. One month after the instruction, a pretest was conducted in order to measure their knowledge and attitude. Three months after the instruction, their practice was measured. The results showed a significant increase in mean scores for K.A.P. from pretest test in the case group as opposed to the control group in which no significant increase was observed. to post Objective measures in the case group after the instruction showed a significant increase in the mean scores of number of ups (p=0.000), duration of group instruction (p=0.001), number of group instruction (p=0.003), duration of face to follow face instruction (p=0.000), and quality of face to face instruction (p=0.000). The findings also showed a significant relationship between health workers" age and their practice before instruction (p=0.01, r=0.3). There was also a meaningful relationship between the duration of their work experience and practice before instruction (p=0.02, r=0.3).