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Information Journal Paper

Title

COMPARISON OF SURVIVAL RATE OF FREEZED- THAWED EJACULATED SPERM, PESA AND TESE WITHIN EMPTY ZONA PELLUCIDA

Pages

  51-56

Abstract

 Cryopreservation of single human SPERMatozoa is a new technique to improve the management of male infertility which first introduced by Cohen et al. (1997) and was repored by the others. The aim of this research was to examine the efficiency of this CRYOPRESERVATION method for the SPERM with different sources (ejaculated SPERM- PESA-TESE). SPERMatozoa was obtained from the patients attending the ICSI program at Koassar Fertility Center and was divided into three groups: a-ejaculated SPERM b-PESA c-TESE (both with shaking SPERM). To every group, 10 empty zonae were prepared by using degenerated, immature or unfertilized human oocytes. 8-10 spelmatozae were transferred into every empty zona. The zonae were put in a droplet of follicular fluid + 15% glycerol (V/V) for five minutes and after loading in a 0.25µl straw plunged in liquid nitrogen. After thawing removal of the SPERMatozae from the zonae were performed and the viability and motility of the SPERMs were recorded and analyzed statistically. The results showed that less than 12% of the SPERMs was lost prior CRYOPRESERVATION and there was no significant difference between groups. Post thaw viability rates did not differ between groups and it was 80-84%. Mortality rate was 70-74% with no significant difference between groups. This SPERM CRYOPRESERVATION is suitable for olygoazooSPERMia patients, because of high recovery and motility rates. It can help the mentioned patients by prevention of repeating surgeries.  

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    APA: Copy

    MOVAHEDIN, M.. (2002). COMPARISON OF SURVIVAL RATE OF FREEZED- THAWED EJACULATED SPERM, PESA AND TESE WITHIN EMPTY ZONA PELLUCIDA. DANESHVAR MEDICINE, 9(40), 51-56. SID. https://sid.ir/paper/30986/en

    Vancouver: Copy

    MOVAHEDIN M.. COMPARISON OF SURVIVAL RATE OF FREEZED- THAWED EJACULATED SPERM, PESA AND TESE WITHIN EMPTY ZONA PELLUCIDA. DANESHVAR MEDICINE[Internet]. 2002;9(40):51-56. Available from: https://sid.ir/paper/30986/en

    IEEE: Copy

    M. MOVAHEDIN, “COMPARISON OF SURVIVAL RATE OF FREEZED- THAWED EJACULATED SPERM, PESA AND TESE WITHIN EMPTY ZONA PELLUCIDA,” DANESHVAR MEDICINE, vol. 9, no. 40, pp. 51–56, 2002, [Online]. Available: https://sid.ir/paper/30986/en

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