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Information Journal Paper

Title

DESIGN AND FABRICATION OF ANATOMICAL BIOREACTOR SYSTEMS CONTAINING ALGINATE SCAFFOLDS FOR CARTILAGE TISSUE ENGINEERING

Pages

  65-74

Abstract

 through alginate gel molding to mimic cartilage tissue in a three-dimensional culture system. The perfusion biomimetic bioreactor was designed to mimic natural joint. The shear stresses exerting on the bioreactor chamber were calculated by Computational Fluid Dynamic (CFD). Several alginate/bovine chondrocyte constructs were prepared, and were cultured in the bioreactor.Histochemical and immunohistochemical staining methods for the presence of glycosaminoglycan (GAG), overall matrix production and type II collagen protein were performed, respectively. The dynamic mechanical device applied a linear mechanical displacement of 2 mm to 10 mm. The CFD modeling indicated peak velocity and maximum wall shear stress were 1.706×10-3 m/s and 0.02407 dyne/cm2, respectively. Histochemical and immunohistochemical analysis revealed evidence of cartilage-like tissue with lacunas similar to those of natural cartilage and the production of sulfated GAG of matrix by the chondrons, metachromatic territorial matrix-surrounded cells and accumulation of type II collagen around the cells. The present study indicated that when chondrocytes were seeded in alginate hydrogel and cultured in biomimetic cell culture system, cells survived well and secreted newly synthesized matrix led to improvement of chondrogenesis.

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    APA: Copy

    MOHAMMAD GHARRAVI, ANNEH, ORAZIZADEH, MAHMOUD, ANSARI ASL, KARIM, BANONI, SALEM, IZADI, SINA, & HASHEMITABAR, MAHMOUD. (2012). DESIGN AND FABRICATION OF ANATOMICAL BIOREACTOR SYSTEMS CONTAINING ALGINATE SCAFFOLDS FOR CARTILAGE TISSUE ENGINEERING. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 4(2 (13)), 65-74. SID. https://sid.ir/paper/313778/en

    Vancouver: Copy

    MOHAMMAD GHARRAVI ANNEH, ORAZIZADEH MAHMOUD, ANSARI ASL KARIM, BANONI SALEM, IZADI SINA, HASHEMITABAR MAHMOUD. DESIGN AND FABRICATION OF ANATOMICAL BIOREACTOR SYSTEMS CONTAINING ALGINATE SCAFFOLDS FOR CARTILAGE TISSUE ENGINEERING. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2012;4(2 (13)):65-74. Available from: https://sid.ir/paper/313778/en

    IEEE: Copy

    ANNEH MOHAMMAD GHARRAVI, MAHMOUD ORAZIZADEH, KARIM ANSARI ASL, SALEM BANONI, SINA IZADI, and MAHMOUD HASHEMITABAR, “DESIGN AND FABRICATION OF ANATOMICAL BIOREACTOR SYSTEMS CONTAINING ALGINATE SCAFFOLDS FOR CARTILAGE TISSUE ENGINEERING,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 4, no. 2 (13), pp. 65–74, 2012, [Online]. Available: https://sid.ir/paper/313778/en

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