COMBINED TREATMENT OF ANDROGEN-INDEPENDENT PROSTATE CANCER CELL LINE DU145 WITH CHEMOTHERAPEUTIC AGENTS AND LITHIUM CHLORIDE: EFFECT ON GROWTH ARREST AND/OR APOPTOSIS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HOSSEIN GHAMARTAJ; AZIMIAN ZAVAREH VAJIHE; SAHRANAVARD FARD PARISSA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY Year:2012 | Volume:4 | Issue:2 (13) Page(s): 75-87

Download Full-Text

View:

379

Download:

148

Cites:

Information Journal Paper

Title

COMBINED TREATMENT OF ANDROGEN-INDEPENDENT PROSTATE CANCER CELL LINE DU145 WITH CHEMOTHERAPEUTIC AGENTS AND LITHIUM CHLORIDE: EFFECT ON GROWTH ARREST AND/OR APOPTOSIS

Author(s)

HOSSEIN GHAMARTAJ | AZIMIAN ZAVAREH VAJIHE | SAHRANAVARD FARD PARISSA | Issue Writer Certificate

Keywords

ANTINEOPLASTIC AGENTS

COMBINATION THERAPY

GSK-3β

LITHIUM CHLORIDE

P53

Abstract

Hormone-independent prostate cancer cell lines are resistant to antineoplastic drugs, this study sought to determine the usefulness of lithium chloride as an inhibitor of glycogen synthase kinase-3b to increase the cytotoxic effect of doxorubicin, etoposide or vinblastine antineoplastic drugs on DU145 cells.Combination effect was assessed by using low and IC50 doses of drugs+lithium chloride. Subsequently, cell cycle analysis and p53 levels and its subcellular localization as a key regulator of cell cycle were assessed. Lithium chloride showed cytotoxic effect in a dose and time dependent manner (p<0.001).Both drugs doxorubicin and etoposide in combination with lithium chloride (LiCl) showed higher percent of cells in SubG1 compared to control (p<0.001).Combination of IC50 dose of doxorubicin and lithium chloride led to S phase arrest (p<0.001, compared to control, lithium chloride or doxorubicin alone).Moreover, G2/M arrest was significantly increased when low dose of doxorubicin and vinblastine were combined with lithium chloride (p<0.001, compared to control and lithium chloride alone). DU145 cells were highly sensitive to vinblastine and no significant changes were observed when combined with lithium chloride. The IC50 doses of all three drugs combined with lithium chloride demonstrated decreased cell percent in G1 phase compared to control or lithium chloride alone (p<0.001). Moreover, in the presence of lithium chloride there were increased levels of p53 in cytoplasm and nucleus (p<0.05). Our results suggest that combination of lithium chloride with chemotherapeutic agents may increases their cytotoxic effect on hormone non-responsive human prostate cancer cells.

Multimedia

No record.

Cites

No record.

References

No record.

Cite

APA: Copy

HOSSEIN, GHAMARTAJ, AZIMIAN ZAVAREH, VAJIHE, & SAHRANAVARD FARD, PARISSA. (2012). COMBINED TREATMENT OF ANDROGEN-INDEPENDENT PROSTATE CANCER CELL LINE DU145 WITH CHEMOTHERAPEUTIC AGENTS AND LITHIUM CHLORIDE: EFFECT ON GROWTH ARREST AND/OR APOPTOSIS. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 4(2 (13)), 75-87. SID. https://sid.ir/paper/313780/en

Vancouver: Copy

HOSSEIN GHAMARTAJ, AZIMIAN ZAVAREH VAJIHE, SAHRANAVARD FARD PARISSA. COMBINED TREATMENT OF ANDROGEN-INDEPENDENT PROSTATE CANCER CELL LINE DU145 WITH CHEMOTHERAPEUTIC AGENTS AND LITHIUM CHLORIDE: EFFECT ON GROWTH ARREST AND/OR APOPTOSIS. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2012;4(2 (13)):75-87. Available from: https://sid.ir/paper/313780/en

IEEE: Copy

GHAMARTAJ HOSSEIN, VAJIHE AZIMIAN ZAVAREH, and PARISSA SAHRANAVARD FARD, “COMBINED TREATMENT OF ANDROGEN-INDEPENDENT PROSTATE CANCER CELL LINE DU145 WITH CHEMOTHERAPEUTIC AGENTS AND LITHIUM CHLORIDE: EFFECT ON GROWTH ARREST AND/OR APOPTOSIS,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 4, no. 2 (13), pp. 75–87, 2012, [Online]. Available: https://sid.ir/paper/313780/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops