مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

EFFECT OF HEPATITIS B VIRUS X GENE ON THE EXPRESSION LEVEL OF P53 GENE USING HEP G2 CELL LINE

Pages

  3-9

Abstract

 Background: The HBV-X (HBX) protein is believed to contribute to the development of HCC. However, the molecular mechanisms involved in HBX-mediated hepatocarcinogenesis remain obscure. In this study, the effect of HEPATITIS B VIRUS X GENE and its protein product HBxAg on expression of P53 GENE in HEP G2 CELL LINE was investigated.Methods: Viral DNA extracted from HBV-positive serum and HBX gene re-gion was amplified using polymerase chain reaction (PCR). Then, PCR prod-uct was cloned into the pcDNA3 vector. After confirmation of cloning, the recombinant plasmid pcDNA3-X was transfected into HepG2 cell line using lipid-mediated DNA-transfection procedure. SDS-PAGE and western blotting methods were used to identify expression of HBX protein. Relative quantification was used to analyze the p53gene expression using the 2-DD Ct method.Results: Recombinant plasmid pcDNA3–HBX was confirmed by restriction endonucleases digestion and colony-PCR. The results of SDS-PAGE and western blot assays showed that HBX gene could be expressed in HEP G2 CELL LINE. There was no significant difference between the expression levels of p53 compared with GAPDH gene as housekeeping gene (p<0.05).Conclusion: There was no significant difference in the protein levels between the transfected cells with X GENE containing HBX130 and HBX131 double mu-tations and P53 GENE. It is necessary to do more studies on HEPATITIS B VIRUS to understand the role of HBX on the development of liver cancer and its function on p53 tumor suppressor protein.

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    • References

    Cite

    APA: Copy

    KORDESTANI, ROGHYEH, MIRSHAFIEE, HAMIDEH, HOSSEINI, SEYED MASOUD, & SHARIFI, ZOHREH. (2014). EFFECT OF HEPATITIS B VIRUS X GENE ON THE EXPRESSION LEVEL OF P53 GENE USING HEP G2 CELL LINE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 6(1), 3-9. SID. https://sid.ir/paper/313822/en

    Vancouver: Copy

    KORDESTANI ROGHYEH, MIRSHAFIEE HAMIDEH, HOSSEINI SEYED MASOUD, SHARIFI ZOHREH. EFFECT OF HEPATITIS B VIRUS X GENE ON THE EXPRESSION LEVEL OF P53 GENE USING HEP G2 CELL LINE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2014;6(1):3-9. Available from: https://sid.ir/paper/313822/en

    IEEE: Copy

    ROGHYEH KORDESTANI, HAMIDEH MIRSHAFIEE, SEYED MASOUD HOSSEINI, and ZOHREH SHARIFI, “EFFECT OF HEPATITIS B VIRUS X GENE ON THE EXPRESSION LEVEL OF P53 GENE USING HEP G2 CELL LINE,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 6, no. 1, pp. 3–9, 2014, [Online]. Available: https://sid.ir/paper/313822/en

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